Anti-DENR Polyclonal Antibody for WB, IHC-P, ELISA - O43583

Core Product Specifications and Parameters

Biological Background: DENR Function and Localization

• DENR (Density-regulated protein), also known as DRP1 and SMAP-3, is a translation regulator that forms a complex with MCTS1 to promote translation reinitiation.
• The MCTS1/DENR complex facilitates dissociation of deacylated tRNAs from post-termination 40S ribosomal complexes and recruits aminoacylated initiator tRNA to the P site in an EIF2-independent manner.
• This reinitiation mechanism governs the translation of over 150 genes that depend on the MCTS1/DENR complex for efficient protein synthesis.
• DENR is predominantly localized in the cytoplasm, consistent with its role in ribosomal recycling and translation initiation.
• The protein is subject to post-translational modifications including acetylation and phosphorylation, which may regulate its activity or interactions.
• Tissue expression is high in heart and skeletal muscle, moderate in brain, placenta, liver, and pancreas, and weak in lung and kidney, suggesting tissue-specific regulation of translation reinitiation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1-198 of human DENR, covering a region that is well-conserved across species (human, mouse, rat), supporting multi-species reactivity.
• For Western blot, the predicted molecular weight is approximately 22 kDa; consider using appropriate positive controls (e.g., heart or skeletal muscle lysates) and validate band specificity.
• In immunohistochemistry (IHC-P), antigen retrieval may be required; test on tissues known to express DENR, such as heart or skeletal muscle, and include negative controls.
• For ELISA applications, pairing with a validated capture antibody may improve sensitivity; perform titration experiments to determine optimal working concentrations.
• Always validate the antibody in your specific sample system and application, as performance can vary with experimental conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For studies involving translation regulation or ribosome recycling, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. Our supply capabilities ensure you obtain consistent, high-quality reagents to support your research and diagnostic development needs.