Anti-DENN (MADD) Monoclonal Antibody for WB, IHC-P, ELISA - Q8WXG6

Core Product Specifications and Parameters

Biological Background: DENN (MADD) Function and Localization

• MADD (MAP kinase-activating death domain protein), also known as DENN, IG20, or Rab3GEP, is a guanine nucleotide exchange factor (GEF) for small Rab GTPases. It specifically activates RAB27A/RAB27B and RAB3A/RAB3C/RAB3D by converting them from GDP-bound inactive to GTP-bound active forms. Related references: PMID:18559336 PMID:20937701

• Plays a critical role in synaptic vesicle exocytosis and trafficking. It is involved in synaptic vesicle formation and function at the neuromuscular junction, and mediates motor-dependent transport of GTP-RAB3A-positive vesicles to presynaptic terminals via interaction with KIF1B and KIF1A.

• Participates in TNFA-mediated activation of the MAPK/ERK pathway. It links TNFRSF1A to MAP kinase activation and may regulate TNFA-induced apoptosis. Related references: PMID:32761064 PMID:9115275 PMID:11577081

• Subcellular localization includes the cell membrane, cytoplasm, and axonal cell projections, consistent with its roles in membrane trafficking and signaling.

• Exhibits tissue-specific expression with multiple isoforms due to alternative splicing. Detected in brain, heart, testis, ovary, spleen, thymus, prostate, small intestine, colon, and liver, among other tissues.

• Keywords from UniProt highlight involvement in apoptosis, alternative splicing, phosphoprotein signaling, and association with intellectual disability.

• The full-length protein has a molecular weight of approximately 183 kDa.

Experimental Guidance and Technical Tips

• Due to the high molecular weight (~183 kDa), ensure appropriate SDS-PAGE gel percentage (e.g., 6-8% resolving gel) and extended transfer times for Western blot.
• For immunohistochemistry (IHC-P), optimize antigen retrieval conditions and validate staining in positive control tissues such as brain or testis, which express MADD.
• In ELISA applications, the immunogen peptide (aa 1548-1647) can serve as a positive control to confirm antibody binding.
• Be aware of multiple isoforms generated by alternative splicing; band patterns in Western blot may vary across tissues and cell lines.
• As a rabbit monoclonal antibody, pair with suitable anti-rabbit secondary reagents and consider its use in multiplexed detection panels.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a comprehensive one-stop sourcing solution for IVD raw materials. This DENN (MADD) monoclonal antibody supports critical studies in synaptic biology, cell signaling, and apoptosis research. Beyond this product, CamelBio streamlines procurement of validated antibody pairs, optimized recombinant proteins, bulk ancillary reagents, and even rare-target raw materials—bridging every step from concept to clinical application with consistent quality and supply reliability.