Anti-Delta-sarcoglycan (SGCD) Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q92629

Core Product Specifications and Parameters

Biological Background: Delta-sarcoglycan Function and Localization

• Delta-sarcoglycan (SGCD) is a component of the sarcoglycan complex, which is a subcomplex of the dystrophin-glycoprotein complex (DGC). This complex forms a critical link between the F-actin cytoskeleton and the extracellular matrix in muscle cells.
• It is also known as 35 kDa dystrophin-associated glycoprotein (35DAG).
• The SGCD gene encodes a protein that is most strongly expressed in skeletal and cardiac muscle, with lower levels in smooth muscle, brain, and lung.
• Subcellularly, delta-sarcoglycan is localized at the cell membrane, specifically the sarcolemma in muscle cells, and associates with the cytoskeleton.
• It contains a signal-anchor, a transmembrane helix, and disulfide bonds, marking it as a transmembrane glycoprotein.
• Mutations in SGCD are associated with limb-girdle muscular dystrophy type 2F (LGMD2F) and dilated cardiomyopathy, highlighting its role in muscle integrity.
• Alternative splicing of SGCD produces multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen used for this antibody spans amino acids 61-290, which covers a significant portion of the protein; consider this when designing epitope-specific experiments.
• For Western blot (WB), the predicted molecular weight is approximately 32 kDa; ensure appropriate gel percentage and transfer conditions.
• For immunohistochemistry (IHC-P), validate in skeletal or cardiac muscle tissues where expression is highest; include appropriate antigen retrieval methods.
• For ELISA, this antibody may be used as a capture or detection reagent; pair with a suitable matched antibody for optimal sensitivity.
• Since cross-reactivity is noted for human, mouse, and rat, validate in the specific species and tissue of interest.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with comprehensive access to IVD raw materials, technical services, and consulting. This anti-delta-sarcoglycan antibody supports investigations into sarcoglycan complex function and related muscular dystrophies. Beyond this product, CamelBio offers validated antibody pairs, ancillary reagents, and sourcing for rare targets to accelerate your assay development.