Anti-Delta-sarcoglycan (SGCD) Rabbit Monoclonal Antibody for WB, ELISA - Q92629

Core Product Specifications and Parameters

Biological Background: Delta-sarcoglycan Function and Localization

• Delta-sarcoglycan (SGCD) is a 35 kDa dystrophin-associated glycoprotein, also known as 35DAG, encoded by the SGCD gene (Gene ID: 6444) in humans.
• It is a component of the sarcoglycan complex, a subcomplex of the dystrophin-glycoprotein complex (DGC), which connects the intracellular F-actin cytoskeleton to the extracellular matrix, essential for muscle fiber stability and integrity.
• Mutations in SGCD are associated with limb-girdle muscular dystrophy type 2F (LGMD2F) and dilated cardiomyopathy, highlighting its critical role in cardiac and skeletal muscle function.
• The protein localizes primarily to the sarcolemma (cell membrane) in muscle cells, with additional distribution in the cytoplasm and along the cytoskeleton.
• Delta-sarcoglycan is a transmembrane glycoprotein with a signal-anchor, a single transmembrane helix, and a disulfide bond, features critical for its structural role.
• Expression is predominantly in skeletal and cardiac muscle, with moderate to high levels in smooth muscle, and lower levels in brain and lung.
• The SGCD gene undergoes alternative splicing, producing isoforms that may have tissue-specific roles or regulation.

Experimental Guidance and Technical Tips

• The antibody was raised against a synthetic peptide spanning amino acids 190-289 of human Delta-sarcoglycan. This region lies within the extracellular domain, suitable for detecting both membrane-bound and soluble forms if present.
• For Western blot, the predicted molecular weight is ~32 kDa. Consider loading appropriate positive controls such as human skeletal muscle or heart lysates, where SGCD is highly expressed.
• In ELISA, the peptide immunogen can be used as a positive control to verify antibody reactivity.
• Cross-reactivity with mouse is reported; thus, appropriate blocking and detection conditions should be optimized for mouse samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio accelerates your IVD development for muscular dystrophy and sarcolemma research by offering one-stop access to high-quality raw materials, including validated antibody pairs and optimized monoclonal antibodies like this anti-SGCD clone. From bench to clinic, we source rare targets, bulk ancillary reagents, and provide technical consulting to streamline your diagnostic pipeline.