Anti-Delta-sarcoglycan (SGCD) Polyclonal Antibody for WB, IF/ICC, ELISA - Q92629

Core Product Specifications and Parameters

Biological Background: Delta-sarcoglycan (SGCD) Function and Localization

• Delta-sarcoglycan (SGCD) is also known as 35 kDa dystrophin-associated glycoprotein (35DAG) and is a component of the sarcoglycan complex.
• The sarcoglycan complex is a subcomplex of the larger dystrophin-glycoprotein complex, which mechanically links the F-actin cytoskeleton to the extracellular matrix, primarily in muscle cells.
• SGCD is most strongly expressed in skeletal and cardiac muscle, with lower expression in smooth muscle, and weak expression in brain and lung.
• Subcellularly, delta-sarcoglycan localizes to the cell membrane, specifically the sarcolemma in muscle cells, and is associated with the cytoskeleton.
• The protein is a transmembrane glycoprotein with a signal-anchor and contains disulfide bonds; it undergoes alternative splicing.
• Mutations in SGCD are associated with cardiomyopathy and limb-girdle muscular dystrophy (LGMD), classifying it as a disease variant protein.

Experimental Guidance and Technical Tips

• The immunogen targets amino acids 61-290 of human delta-sarcoglycan, a region that may include extracellular or intracellular domains; consider this when designing experimental protocols.
• For Western blot (WB), the predicted molecular weight is approximately 32kDa; include appropriate positive controls such as skeletal or cardiac muscle lysates from human, mouse, or rat.
• In immunofluorescence/immunocytochemistry (IF/ICC), validate the antibody’s localization to the sarcolemma using muscle tissue sections or appropriate cell lines.
• For ELISA, optimize coating concentration and detection conditions empirically, as no standardized dilution is provided.
• Given cross-reactivity with mouse and rat, this antibody can potentially be used in comparative studies; however, always validate specificity in your own sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a streamlined procurement channel for IVD raw materials, from validated antibodies like this delta-sarcoglycan polyclonal antibody to bulk ancillary reagents and rare target sourcing. This product is ideal for studying sarcoglycan complex involvement in muscular dystrophy and cardiomyopathy. Partner with us to efficiently bridge your research and diagnostic development needs.