Anti-DEGS1 Rabbit Polyclonal Antibody for WB, ELISA - O15121

Core Product Specifications and Parameters

Biological Background: DEGS1 Function and Localization

• DEGS1 (Sphingolipid delta(4)-desaturase DES1) is also known as DES1, MLD, MIG15, and FADS7, and belongs to the membrane fatty acid desaturase family.
• It catalyzes the conversion of D-erythro-sphinganine to D-erythro-sphingosine, a key step in sphingolipid biosynthesis. Related references: PMID:11937514 PMID:30620337 PMID:30620338
• Additionally, DEGS1 can act as a retinol isomerase, catalyzing equilibrium isomerization of retinols (By similarity).
• The protein localizes to both the mitochondrial membrane and the endoplasmic reticulum membrane, reflecting its role in lipid metabolism.
• DEGS1 is ubiquitously expressed in human tissues, indicating its fundamental role in cellular sphingolipid homeostasis.
• Post-translational modifications include phosphorylation and potential N-myristoylation, which may regulate its activity or membrane association.
• Mutations in DEGS1 are associated with leukodystrophy, highlighting its clinical significance in neurological disorders.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 234-323 near the C-terminus of human DEGS1; this region is predicted to be intracellular, so the antibody is expected to detect the native protein.
• For Western blot, consider using membrane protein extraction protocols and ensure sufficient denaturation to resolve the ~38 kDa band.
• In ELISA applications, validate the antibody in the appropriate sample matrix to confirm sensitivity and specificity.
• As a polyclonal antibody, it may recognize multiple epitopes; consider pre-adsorption if cross-reactivity is a concern.
• The antibody was raised in rabbit; ensure compatibility with secondary detection reagents for your assay system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and labs with one-stop access to IVD raw materials, including validated antibodies like this anti-DEGS1 polyclonal antibody for sphingolipid metabolism research. Our supply capabilities cover optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and sourcing for rare targets, supporting your workflow from concept to clinic.