Anti-DEFB4A (Beta-defensin 2) Rabbit Polyclonal Antibody for WB, IF-P, ELISA - O15263

Core Product Specifications and Parameters

Biological Background: DEFB4A (Beta-defensin 2) Function and Localization

• DEFB4A, also known as beta-defensin 2 (BD-2, hBD-2), SAP1, DEFB2, DEFB4, DEFB102, is a member of the beta-defensin family of antimicrobial peptides.
• Exhibits antimicrobial activity against Gram-negative and Gram-positive bacteria, with highest activity against Gram-negative bacteria (PubMed:10837369, PubMed:9202117). Related references: PMID:10837369, PMID:9202117
• Its antimicrobial activity against P. aeruginosa is salt-sensitive and reduced in high salt concentrations greater than 25 mM (PubMed:10837369). Related references: PMID:10837369
• Also exhibits antifungal activity against C. albicans, permeabilizing cell membranes by targeting plasma membrane lipid phosphatidylinositol 4,5-bisphosphate (PIP2) (PubMed:30050988, PubMed:10837369, PubMed:9202117). Related references: PMID:30050988, PMID:10837369, PMID:9202117
• Acts as a ligand for C-C chemokine receptor CCR6, inducing chemotaxis of immature dendritic cells and memory T cells (PubMed:10521347, PubMed:20068036). Related references: PMID:10521347, PMID:20068036
• DEFB4A is a secreted protein, detectable in epithelial lining fluid and extracellular spaces.
• Expressed predominantly in lung epithelial cells, foreskin, lung, and trachea, with lower expression in kidney, uterus, and salivary gland (PubMed:10837369, PubMed:9202117, PubMed:9831658). Related references: PMID:10837369, PMID:9202117, PMID:9831658
• Contains conserved cysteine residues forming intramolecular disulfide bonds, characteristic of defensins, contributing to structural stability and antimicrobial function.

Experimental Guidance and Technical Tips

• The immunogen used is a synthetic peptide corresponding to amino acids 1-64 of human DEFB4A, which covers the N-terminal region including the signal peptide. Consider that the antibody may recognize the precursor and/or mature forms; validation in relevant biological systems is recommended.
• For Western blot (WB), due to the small molecular weight of ~7 kDa, use a high-percentage polyacrylamide gel (e.g., 15% or gradient gel) and carefully optimize transfer conditions to avoid over-transfer. Positive controls can include lysates from human lung or tracheal epithelial cells.
• In immunofluorescence on paraffin-embedded sections (IF-P), anticipate extracellular or vesicular staining patterns, as DEFB4A is a secreted protein. Permeabilization may be adjusted; include appropriate controls.
• For ELISA, the antibody can be used for detection of native or recombinant DEFB4A in biological fluids such as bronchoalveolar lavage or cell culture supernatants. Pairing with a capture antibody may be explored for sandwich ELISA development.
• This antibody is reactive with human DEFB4A; cross-reactivity with other species or defensin family members has not been tested as per available data. Validate specificity in your sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing partner for IVD raw materials, supporting diagnostic manufacturers and research laboratories from concept to clinic. This anti-DEFB4A polyclonal antibody is part of our extensive portfolio for antimicrobial peptide and innate immunity research. Through our global supply chain, we offer access to validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and sourcing solutions for rare targets, all backed by efficient logistics and technical support.