Anti-DEFB132 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q7Z7B7

Core Product Specifications and Parameters

Biological Background: DEFB132 Function and Localization

• DEFB132 (beta-defensin 132) is a member of the beta-defensin family of small cationic antimicrobial peptides, also designated BD-32, DEFB32, or HEL-75.
• The protein functions as an antimicrobial agent with demonstrated antibacterial activity.
• As a secreted peptide, DEFB132 localizes extracellularly after cleavage of its N-terminal signal peptide.
• The mature peptide contains a characteristic defensin fold stabilized by disulfide bonds, a hallmark of this family.
• UniProt keywords include Antibiotic, Antimicrobial, Disulfide bond, Secreted, and Signal, which underscore its role in host defense.
• The protein is encoded by the DEFB132 gene (Gene ID: 400830) on human chromosome 20 and is part of a larger defensin cluster with similar genomic organization.
• While no specific tissue distribution is reported, defensins are commonly expressed in epithelial tissues and immune cells as part of innate immunity.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 1–95 of the full-length DEFB132 protein, including both the signal sequence and mature peptide; consider possible cross-reactivity with unprocessed precursors in certain sample types.
• For Western blotting, the predicted molecular weight is approximately 11 kDa; use appropriate low-molecular-weight markers and consider reducing conditions to resolve disulfide-bridged forms.
• In immunohistochemistry (IHC-P), optimal antigen retrieval protocols should be empirically determined; include positive control tissues known to express defensins, such as skin or mucosal epithelium.
• ELISA conditions may require careful titration of antibody and detection reagents; validate in the relevant biological matrix to ensure specificity.
• The antibody cross-reacts with mouse DEFB132, enabling translational studies; however, confirm cross-reactivity in your mouse strain or tissue of interest.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted one-stop partner for diagnostic manufacturers and research laboratories seeking IVD raw materials. For antimicrobial peptide research and innate immunity applications, CamelBio offers validated polyclonal antibodies, optimized antibody pairs, and bulk ancillary reagents. Our sourcing capabilities extend to rare targets like beta-defensins, ensuring you get consistent, high-quality materials for every stage of assay development.