Anti-DEFA5/Defensin alpha 5 Polyclonal Antibody for WB, ELISA - Q01523

Core Product Specifications and Parameters

Biological Background: DEFA5 Function and Localization

• DEFA5 (Defensin alpha 5, HD5) is a host-defense peptide that maintains sterility in the urogenital system. Related references: PMID:12021776, PMID:12660734, PMID:15616305
• It exhibits antimicrobial activity against a broad spectrum of bacteria, including Gram-negative E. coli, P. aeruginosa, S. typhimurium, and Gram-positive S. aureus, B. cereus, L. monocytogenes, among others. Related references: PMID:12021776, PMID:15616305, PMID:19589339
• DEFA5 confers resistance to intestinal infection by S. typhimurium and also shows activity against enteric commensal bacteria such as B. adolescentis and L. acidophilus. Related references: PMID:12660734, PMID:25354318
• The peptide binds to bacterial membranes, causing membrane disintegration, and neutralizes B. anthracis lethal factor enzymatic activity. Related references: PMID:25782105, PMID:22573326
• It induces secretion of the chemokine IL-8 by intestinal epithelial cells. Related references: PMID:19589339
• As a defensin, DEFA5 is a secreted protein found in cytoplasmic vesicles and secretory vesicles (UniProt Q01523).
• Tissue expression is prominent in Paneth cells of the small intestine, urothelium, kidney collecting tubules, and female genital tract epithelia; endometrial expression varies with the menstrual cycle. Related references: PMID:12021776, PMID:1429669, PMID:22359618, PMID:9588893
• The mature peptide is a glycoprotein with multiple disulfide bonds, belonging to the defensin family, and functions in innate immunity as an antibiotic and antimicrobial agent (Keywords: Antibiotic, Antimicrobial, Defensin, Disulfide bond, Glycoprotein).

Experimental Guidance and Technical Tips

• For Western blotting, consider using high-percentage SDS-PAGE gels and low molecular weight markers due to the small ~10kDa size of DEFA5.
• Validate antibody specificity in lysates from DEFA5-expressing tissues such as intestinal Paneth cells, urogenital epithelia, or relevant cell lines.
• For ELISA applications, the antibody can be used as a capture or detection reagent; optimize pairing with a matched antibody pair for sandwich ELISA development.
• Since the immunogen corresponds to the mature peptide region (aa 20-94), the antibody is expected to recognize processed and secreted forms of DEFA5.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, labs, and research institutes a streamlined sourcing solution for IVD raw materials. This anti-DEFA5 polyclonal antibody supports research into innate immunity and host-defense mechanisms, complementing our portfolio of validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. Whether you need single reagents or integrated supply chains from concept to clinic, CamelBio bridges your procurement needs with reliable, high-quality materials.