Anti-DEFA1 Polyclonal Antibody for WB, IF/ICC, ELISA - P59665

Core Product Specifications and Parameters

Biological Background: DEFA1 Function and Localization

• Neutrophil defensin 1 (DEFA1), also known as HNP-1, is a member of the alpha-defensin family encoded by the DEFA1 and DEFA1B genes.
• Functions as an effector molecule of the innate immune system with broad antibiotic activity against bacteria, fungi, and viruses (PubMed:15616305, PubMed:17142766, PubMed:20220136). Related references: PMID:15616305 PMID:17142766 PMID:20220136
• Inhibits bacterial cell wall synthesis by interacting with lipid II, an essential precursor of peptidoglycan synthesis (PubMed:20214904). Related references: PMID:20214904
• Blocks adenovirus infection by preventing viral disassembly at the vertex region, thus restricting the release of internal capsid protein pVI required for endosomal membrane penetration (PubMed:18191790). Related references: PMID:18191790
• Promotes a NLRP3-mediated inflammasome response and interleukin-1beta (IL-1beta) release upon interaction with adenovirus capsid, redirecting viral particles to TLR4 (PubMed:35080426). Related references: PMID:35080426
• Induces production of proinflammatory cytokines, including type I interferon (IFN) in plasmacytoid dendritic cells (pDCs), by triggering degradation of NFKBIA and nuclear translocation of IRF1 (PubMed:27031443). Related references: PMID:27031443
• Subject to post-translational modifications such as ADP-ribosylation and phosphorylation, as identified by proteomics.
• Localized as a secreted protein, consistent with its role in extracellular defense.

Experimental Guidance and Technical Tips

• The immunogen design, spanning the full-length mature peptide (aa 1-94), may contribute to the observed cross-reactivity with human, mouse, and rat DEFA1 orthologs.
• For Western blot (WB), consider optimizing primary antibody dilution to detect the low-molecular-weight target (10 kDa). Include appropriate blocking and washing steps to minimize background.
• For immunofluorescence/immunocytochemistry (IF/ICC), validate reactivity in relevant cell types such as neutrophils or other myeloid cells known to express DEFA1.
• When performing ELISA, ensure the recombinant or native defensin standards match the expected molecular form (e.g., processed mature peptide).
• Cross-reactivity with mouse and rat allows for potential use in translational models, but users should verify specificity in the relevant sample systems.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research labs a one-stop sourcing platform for IVD raw materials, including validated antibodies for innate immunity targets like DEFA1. Our supply capabilities encompass optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and support for rare-target raw-material procurement. Whether you need standard catalog items or customized sourcing solutions, CamelBio bridges the gap from concept to clinic.