Anti-Decorin Rabbit Monoclonal Antibody for WB, IF-P, IHC-P, ELISA - P07585

Core Product Specifications and Parameters

Biological Background: Decorin Function and Localization

• Decorin, also known as Bone proteoglycan II (PG-S2, PG40), is a secreted small leucine-rich repeat proteoglycan encoded by the human DCN gene.
• It is localized to the extracellular space and extracellular matrix, where it may regulate collagen fibril formation.
• The protein is detected in placenta, cerebrospinal fluid, fibroblasts, and urine, indicating widespread expression and secretion.
• Structurally, it features leucine-rich repeats, disulfide bonds, and glycosylation, classifying it as a proteoglycan.
• Alternative splicing of the DCN gene generates isoforms, contributing to functional diversity.
• It has been linked to corneal dystrophy, highlighting its role in tissue integrity.
• The calculated molecular weight is 39,747 Da, with an observed band around 40 kDa in Western blotting.

Experimental Guidance and Technical Tips

• The immunogen was designed using a recombinant fusion protein covering amino acids 31–359 of human Decorin (NP_001911.1), encompassing the core protein region. This design may facilitate recognition of native and denatured Decorin.
• For Western blotting, a band around 40 kDa is expected; pre-stained markers can be used to confirm molecular weight.
• In immunohistochemistry (paraffin-embedded sections), antigen retrieval may influence staining intensity; consider testing various retrieval methods.
• This antibody has been reported to cross-react with rat Decorin, making it potentially useful for rodent model studies; however, species-specific differences should be validated in the relevant sample system.
• For ELISA applications, the antibody may be used as a capture or detection reagent; validate pairing with compatible secondary antibodies to avoid cross-reactivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive range of IVD raw materials, including validated antibody pairs, optimized monoclonal antibodies like this anti-Decorin clone, and bulk ancillary reagents for diagnostic development. With expertise in rare target sourcing, we support extracellular matrix and proteoglycan research projects from concept to clinic. Contact our team for seamless procurement of high-quality antibodies and custom raw-material solutions.