Anti-Decorin Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P07585

Core Product Specifications and Parameters

Biological Background: Decorin Function and Localization

• Decorin, also known as bone proteoglycan II or PG40, is a secreted proteoglycan encoded by the DCN gene (SLRR1B) in humans. It belongs to the small leucine-rich proteoglycan (SLRP) family.
• The protein features multiple leucine-rich repeats, which mediate protein-protein interactions and contribute to its role in the extracellular matrix.
• Decorin's primary function is the regulation of collagen fibrillogenesis; it may affect the rate of fibril formation.
• It is secreted into the extracellular space and is a component of the extracellular matrix. Tissue expression has been detected in placenta, cerebrospinal fluid, fibroblasts, and urine. Related references: PMID:32337544 PMID:25326458 PMID:36213313 PMID:37453717
• Post-translational modifications include glycosylation and disulfide bond formation. Alternative splicing has also been noted.
• Keywords associated with decorin include: Secreted, extracellular matrix, glycoprotein, leucine-rich repeat, proteoglycan, disulfide bond, and corneal dystrophy (mutations in DCN are linked to congenital stromal corneal dystrophy).

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 31-359 of human decorin (mature protein after signal peptide cleavage), so the antibody may recognize both glycosylated and non-glycosylated forms.
• For Western blotting, a band at approximately 40 kDa is expected; however, post-translational modifications such as glycosylation can cause a shift in molecular weight.
• Polyclonal antibodies can exhibit lot-to-lot variability; consider titrating and validating the antibody in your specific sample type and application.
• Indicated cross-reactivity with mouse and rat; verify reactivity in your species of interest if critical.
• For IHC-P, optimization of antigen retrieval and antibody dilution is recommended.
• In ELISA, pairing with a suitable capture/detection antibody may enhance performance.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated IVD raw material partner, CamelBio offers a reliable supply of antibodies like this polyclonal anti-Decorin to support extracellular matrix and proteoglycan research. We provide one-stop access to validated antibody pairs, optimized monoclonals and polyclonals, bulk ancillary reagents, and sourcing for rare targets, helping diagnostic developers move efficiently from concept to clinic.