Anti-DDX6 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P26196

Core Product Specifications and Parameters

Biological Background: DDX6 Function and Localization

• DDX6 (DEAD-box helicase 6) is a probable ATP-dependent RNA helicase, also known as DEAD box protein 6, oncogene RCK, or p54, involved in RNA metabolism.
• Essential for the formation of P-bodies, cytosolic membrane-less ribonucleoprotein granules that coordinate storage of translationally inactive mRNAs, preventing their degradation.
  Related references: PMID:25995375, PMID:27342281, PMID:31422817
• Plays a role in mRNA decapping during the process of mRNA degradation.
  Related references: PMID:16364915
• Blocks autophagy in nutrient-rich conditions by repressing the expression of ATG-related genes through degradation of their transcripts.
  Related references: PMID:26098573
• Localizes to the cytoplasm, P-bodies, nucleus, and cytoplasmic ribonucleoprotein granules, reflecting its dynamic role in mRNA storage and metabolism.
• Abundantly expressed in most tissues.
• Associated with multiple keywords including ATP-binding, RNA-binding, helicase, phosphoprotein, proto-oncogene, chromosomal rearrangement, and disease variants linked to intellectual disability.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 384-483 of human DDX6, a region conserved in mouse and rat, supporting the observed cross-reactivity.
• For Western blot (WB), a band of approximately 54 kDa is expected; consider using positive control lysates from cell lines with high DDX6 expression.
• In immunohistochemistry (IHC-P), antigen retrieval methods (e.g., citrate buffer, heat-induced) may be necessary; optimization is recommended.
• For immunocytochemistry/immunofluorescence (IF/ICC), permeabilization is required to visualize cytoplasmic and P-body localization.
• ELISA application can be used for titer determination and quantification, but optimal coating concentration should be determined experimentally.
• When investigating P-bodies or stress granules, co-staining with established markers (e.g., DCP1A) could validate target specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering validated antibodies, bulk ancillary reagents, and custom solutions to support diagnostic development and research. This anti-DDX6 polyclonal antibody caters to studies on mRNA metabolism, P-body dynamics, and autophagy-related pathways. Explore our portfolio for one-stop access to high-quality reagents and technical consulting to accelerate your projects.