Anti-DDX56 Polyclonal Antibody for WB, ELISA - Q9NY93

Core Product Specifications and Parameters

Biological Background: DDX56 Function and Localization

• DDX56 (also known as DDX21, NOH61) is a member of the DEAD box family of ATP-dependent RNA helicases, characterized by a conserved Asp-Glu-Ala-Asp (DEAD) motif, and functions in diverse RNA-related processes including ribosome biogenesis and innate immunity.
• It acts as a nucleolar RNA helicase that plays an essential role in maintaining nucleolar integrity, with studies in planarian stem cells highlighting its critical function in this process. Related references: PMID:33789112
• DDX56 positively regulates antiviral innate immunity by inhibiting virus-triggered nuclear translocation of IRF3. It disrupts the IRF3-importin IPO5 interaction, thereby preventing IRF3-mediated signaling. Related references: PMID:31340999
• The helicase is involved in later stages of pre-ribosomal particle processing, contributing to the maturation of 60S ribosomal subunits, and possesses intrinsic ATPase activity.
• DDX56 maintains embryonic stem cell proliferation through conventional regulation of ribosome assembly and interaction with the OCT4/POU5F1 transcriptional complex (By similarity).
• In the context of microbial infections, DDX56's helicase activity is important for packaging viral RNA into virions during West Nile virus infection, and it plays positive roles in foot-and-mouth disease virus and EMCV replication by interrupting IRF3 phosphorylation and nuclear translocation.
• Subcellularly, DDX56 localizes to the nucleolus within the nucleus, consistent with its functions in ribosome biogenesis and nucleolar organization.
• Tissue expression analysis shows DDX56 is detected in various tissues including heart, brain, liver, pancreas, placenta, and lung.

Experimental Guidance and Technical Tips

• The immunogen covers the C-terminal region (amino acids 318‑547) of human DDX56, so the antibody may detect full‑length protein and certain splice variants. For Western blot, the predicted molecular weight is ~62 kDa; validate band size using appropriate positive controls and molecular weight markers.
• This antibody has verified cross‑reactivity with rat, making it suitable for comparative or translational studies across human and rat model systems. Confirm reactivity in your specific sample type before quantitative applications.
• Recommended starting dilutions for WB and ELISA should be optimized empirically. Non‑specific binding may be reduced by adjusting blocking conditions and antibody concentration.
• For ELISA, pairing with a matched detection antibody may improve sensitivity; consider testing both direct and indirect formats.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines procurement for IVD raw materials, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. Our sourcing network supports rare targets like DDX56, enabling research in ribosome biogenesis, innate immunity, and viral pathogenesis. Count on CamelBio for end‑to‑end support from concept to clinic.