Anti-DDX5 Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, IP, ELISA - P17844

Core Product Specifications and Parameters

Biological Background: DDX5 Function and Localization

• DDX5, also known as p68 or HLR1, is a probable ATP-dependent RNA helicase belonging to the DEAD box protein family, playing a central role in RNA processing and transcriptional regulation.
• It is critically involved in alternative pre-mRNA splicing, enhancing tau exon 10 inclusion in an RNA helicase- and RBM4-dependent manner by binding to stem-loop structures downstream of exon 10.
• DDX5 functions as a transcriptional coactivator for multiple factors, including androgen receptor (AR), MYOD1, p53/TP53, and RUNX2, thereby influencing processes like skeletal muscle differentiation, DNA damage response, apoptosis, and osteoblast differentiation.
• In a promoter-specific context, it can also act as a transcriptional repressor, likely via association with histone deacetylases such as HDAC1.
• As a component of the large PER complex, DDX5 participates in circadian rhythm regulation by inhibiting 3' transcriptional termination of clock genes like PER1 and NR1D1.
• The protein is primarily localized to the nucleus, including the nucleolus and nuclear speckles, but can also be detected in the cytoplasm.
• Post-translational modifications such as acetylation, phosphorylation, and ubiquitination (isopeptide bond) have been identified, likely modulating its activity and stability.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the N‑terminus (aa 1–100) of human DDX5, enabling specific detection of the full‑length protein in appropriate sample types.
• In Western blot, expect a band near 69 kDa; optimize antibody dilution and blocking conditions for each sample system to achieve a clear signal.
• For immunohistochemistry (IHC‑P), immunofluorescence (IF/ICC), or immunoprecipitation (IP), consider necessary antigen retrieval or permeabilization steps and validate in the relevant sample.
• The antibody shows cross-reactivity with human, mouse, and rat; if applying to other species, verify reactivity experimentally.
• ATP hydrolysis by DDX5 is strongly stimulated by single‑stranded RNA; this biochemical feature can be leveraged in functional assays when using the antibody for co‑detection or pull‑down.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a one‑stop sourcing solution for IVD raw materials, delivering validated monoclonal and polyclonal antibodies, bulk ancillary reagents, and customized procurement for rare targets. The DDX5 rabbit monoclonal antibody supports researchers and manufacturers developing assays for RNA helicase activity, transcriptional regulation, or cancer biomarker studies. Whether you need matched antibody pairs, optimized clones, or technical consultation for your diagnostic pipeline, CamelBio bridges the gap from concept to clinic.