Anti-DDX5 Polyclonal Antibody for WB, ELISA - P17844

Core Product Specifications and Parameters

Biological Background: DDX5 Function and Localization

• DDX5, also known as p68 RNA helicase, is a member of the DEAD box protein family, characterized by ATP-dependent RNA helicase activity essential for RNA secondary structure modulation.
• Functions in alternative pre-mRNA splicing: its helicase activity promotes tau exon 10 inclusion in an RBM4-dependent manner by binding to the stem-loop region downstream of exon 10.
• Acts as a transcriptional coactivator for multiple transcription factors including androgen receptor (AR), MYOD1 (synergizing with DDX17 and SRA1 RNA), p53/TP53 (involved in DNA damage response and apoptosis), and RUNX2 (regulating osteoblast differentiation).
• Additionally, can function as a transcriptional repressor in a promoter-specific manner, likely through association with histone deacetylases such as HDAC1.
• As a component of the PER complex, DDX5 inhibits 3' transcriptional termination of circadian target genes (e.g., PER1, NR1D1), contributing to circadian rhythm regulation.
• Subcellular localization is primarily nuclear, including nucleolus and nuclear speckles, but it is also detected in the cytoplasm.
• Subject to various post-translational modifications: acetylation, phosphorylation, and ubiquitination (Ubl conjugation), which may regulate its activity and interactions.

Experimental Guidance and Technical Tips

• For Western blot (WB), expect a specific band at approximately 69 kDa. Use standard SDS-PAGE and transfer conditions, and consider validating with DDX5 knockdown lysates or recombinant protein controls.
• The immunogen covers the N-terminal 1-614 amino acid sequence; thus, the antibody may recognize full-length DDX5 and potential isoforms. Verify specificity in your experimental system.
• Cross-reactivity with mouse enables translational studies across species; validate in relevant mouse tissue or cell lines to confirm reactivity.
• For ELISA, optimize coating concentrations and detection methods; the antibody can be used for titer checks or antigen capture assays.
• Given DDX5's roles in splicing and transcription, functional applications like RNA immunoprecipitation (RIP) or co-immunoprecipitation (Co-IP) may be considered, but empirical testing is recommended.
• As a polyclonal antibody, monitor lot-to-lot consistency by including positive and negative controls in each experiment.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, research laboratories, and institutes with a comprehensive one-stop sourcing platform for IVD raw materials. This DDX5 polyclonal antibody supports research in transcriptional regulation, alternative splicing, and circadian biology. Beyond this product, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets to accelerate your assay development from concept to clinic.