Anti-DDX46 Polyclonal Antibody for WB, IHC-P, ELISA - Q7L014

Core Product Specifications and Parameters

Biological Background: DDX46 Function and Localization

• DDX46 (DEAD box protein 46, also known as PRP5/PRPF5) is a probable ATP-dependent RNA helicase and a member of the DEAD-box protein family, characterized by conserved motifs involved in ATP binding and RNA unwinding. Related references: PMID:12234937, PMID:32494006, PMID:34822310
• It is a core component of the 17S U2 small nuclear ribonucleoprotein (snRNP) complex of the spliceosome, the large macromolecular machine that removes introns from pre-mRNAs. Related references: PMID:12234937, PMID:32494006, PMID:34822310
• Within the 17S U2 snRNP, DDX46 directly participates in early spliceosome assembly and mediates recognition of the intron branch site, promoting selection of the branch-site adenosine that serves as the nucleophile for the first catalytic step of splicing. Related references: PMID:32494006, PMID:34822310
• DDX46 is essential for pre-spliceosome assembly and proofreading of the branch site, ensuring accurate and efficient splicing. Related references: PMID:34822310
• Subcellular localization studies indicate that DDX46 is present in nuclear speckles, the nucleoplasm, and Cajal bodies, consistent with its role in pre-mRNA processing and spliceosomal dynamics.
• Post-translational modifications of DDX46 include acetylation, phosphorylation, and ubiquitination, as suggested by high-throughput proteomics data.
• The protein has a calculated molecular weight of approximately 117 kDa and contains an ATP-binding domain, a helicase core, and a C-terminal coiled-coil region, likely mediating protein-protein interactions.

Experimental Guidance and Technical Tips

• Since the immunogen covers the C-terminal region (aa 682–1031), the antibody may detect full-length DDX46 and possibly truncated variants lacking this region. Consider verifying reactivity in your specific sample system.
• DDX46 has a predicted molecular weight of ~117 kDa. For Western blotting, use appropriate gel percentage (e.g., 6–8% Tris-glycine or Bis-Tris gels) and optimize transfer conditions for high-molecular-weight proteins.
• For immunohistochemistry, DDX46’s nuclear localization suggests that permeabilization and antigen retrieval methods may be critical. Validate staining in relevant tissue controls and consider nuclear counterstaining.
• The antibody has been validated for human, mouse, and rat samples. Sequence alignment of DDX46 orthologs may help predict reactivity with other species and guide cross-species experimental design.

CamelBio: Your One-Stop Sourcing Bridge

As a specialized B2B provider of IVD raw materials, CamelBio offers a complete portfolio of validated antibodies, antigen pairs, and ancillary reagents for diagnostic development. This anti-DDX46 polyclonal antibody supports research in spliceosome biology and pre-mRNA processing and is part of our extensive catalog of optimized antibodies for WB, IHC-P, and ELISA applications. Whether you need bulk quantities, custom sourcing for rare targets, or technical consultation from concept to clinic, CamelBio is your trusted partner for one-stop procurement.