Anti-DDX43 Rabbit Polyclonal Antibody for WB and ELISA - Q9NXZ2

Core Product Specifications and Parameters

Biological Background: DDX43 Function and Localization

• DDX43, also known as HAGE or CT13 (Cancer/testis antigen 13), is a probable ATP-dependent RNA helicase belonging to the DEAD box protein family, characterized by the conserved Asp-Glu-Ala-Asp motif.
• As a putative RNA helicase, DDX43 is predicted to utilize ATP hydrolysis to unwind RNA secondary structures, playing roles in RNA metabolism, splicing, translation, and ribosome biogenesis.
• It is classified as a cancer/testis antigen, normally expressed in testis but aberrantly overexpressed in a wide range of tumors at levels up to 100-fold higher than in normal tissues.
• Due to its tumor-restricted expression, DDX43 serves as a potential immunotherapeutic target and biomarker in various cancers.
• The protein contains ATP-binding and RNA-binding domains typical of helicases, enabling its function in nucleotide-dependent RNA unwinding.
• Alternative splicing of the DDX43 gene generates multiple transcript variants, which may influence its expression and functional diversity.
• DDX43 has been identified through proteomics studies, confirming its presence in human protein databases.

Experimental Guidance and Technical Tips

• For Western blot, consider testing a dilution range (e.g., 1:500-1:2000) in lysates from DDX43-expressing tumor cell lines or testis tissue, and validate bands at the expected molecular weight of approximately 73 kDa, keeping in mind possible splice variants.
• For ELISA, pairing with a capture antibody may be needed; the immunogen corresponds to the C-terminal region, so ensure the detection system does not interfere.
• Given cross-reactivity with mouse and rat, this antibody may be suitable for studies in these species, but validation in the relevant sample system is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your comprehensive sourcing partner for IVD raw materials, offering validated antibody pairs, purified polyclonal and monoclonal antibodies, and bulk ancillary reagents. For research on cancer/testis antigens like DDX43, we provide reliable antibody supplies and support with custom sourcing of rare targets to accelerate your diagnostic and therapeutic development programs.