Anti-DDX41 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, IP, ELISA - Q9UJV9

Core Product Specifications and Parameters

Biological Background: DDX41 Function and Localization

• DDX41 (DEAD box protein 41, abstrakt homolog) is a probable ATP-dependent RNA helicase.

• It plays pivotal roles in innate immune sensing and hematopoietic homeostasis by recognizing foreign or self-nucleic acids and initiating anti-pathogen responses.
  Related references:
  PMID:34473945
  PMID:23222971

• Upon phosphorylation by BTK, DDX41 binds dsDNA and interacts with STING1, leading to type I interferon and cytokine responses.
  Related references:
  PMID:25704810
  PMID:35613581

• It modulates dsDNA homeostasis through ATP-dependent unwinding and ATP-independent strand-annealing activities.
  Related references:
  PMID:35613581

• DDX41 regulates alternative splicing of certain immunity-associated genes to control their transcription.
  Related references:
  PMID:33650667

• Binds R-loops to prevent accumulation and maintain genome stability; also participates in pre-mRNA splicing, translation, and snoRNA processing for ribosome biogenesis.
  Related references:
  PMID:36229594
  PMID:36780110

• Localizes to both nucleus and cytoplasm; features include ATP-binding, helicase, zinc-finger, acetylation, and phosphorylation.

Experimental Guidance and Technical Tips

• For western blotting, a band around 70 kDa is expected; validate using positive control lysates from human, mouse, or rat cell lines.
• For immunohistochemistry and immunofluorescence, consider antigen retrieval and permeabilization steps; optimal antibody dilution should be determined experimentally.
• This polyclonal antibody may be suitable for immunoprecipitation and ELISA; performance should be verified in the relevant sample system.
• Batch-to-batch variability is possible; it is recommended to include appropriate controls and validate each new lot.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, providing validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. For research on innate immunity and RNA metabolism like DDX41, we offer one-stop access to high-quality raw materials to support your diagnostic development from concept to clinic.