Anti-DDX4 (VASA) Rabbit Monoclonal Antibody for WB - Q9NQI0

Core Product Specifications and Parameters

Biological Background: DDX4 Function and Localization

• DDX4 (DEAD box protein 4), also known as VASA, is a member of the DEAD-box RNA helicase family, characterized by the conserved Asp-Glu-Ala-Asp (DEAD) motif. It functions as an ATP-dependent RNA helicase essential for germ cell development (PubMed:10920202, PubMed:21034600). Related references: PMID:10920202, PMID:21034600
• In spermatogenesis, DDX4 is required to repress transposable elements via the piRNA pathway, maintaining genomic integrity in the germline (By similarity). This involves forming piRNA-Piwi protein complexes and mediating transposon methylation.
• DDX4 participates in secondary piRNA biogenesis through a ping-pong amplification cycle in fetal male germ cells, and its helicase activity enables processing of pre-piRNAs into mature piRNAs (By similarity).
• The protein contains multiple domains: a central helicase core with ATP-binding and hydrolase activity, and possibly repeats. It undergoes alternative splicing, generating isoforms.
• DDX4 localizes predominantly to the cytoplasm, with enrichment in the perinuclear region (By similarity).
• Tissue expression is restricted to the ovary and testis, with expression in migratory primordial germ cells in the gonadal ridge (By similarity).
• Keywords include developmental protein, differentiation, meiosis, spermatogenesis, and RNA-mediated gene silencing, reflecting its critical roles in germ cell biology.
• There is evidence of phosphorylation as a post-translational modification (from UniProt keyword Phosphoprotein).

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 635–724 near the C-terminus of human DDX4 (NP_077726.1). This region is likely to be specific to human DDX4, but cross-reactivity with mouse and rat has been confirmed. When assessing specificity, consider sequence alignment across species.
• This rabbit monoclonal antibody is suitable for multiple applications: Western blot (WB) detecting a band at ~79 kDa, immunoprecipitation (IP), immunofluorescence on paraffin-embedded tissues (IF-P), immunohistochemistry on paraffin sections (IHC-P), and ELISA. As with any antibody, optimization of dilution and conditions is recommended.
• For studies involving germ cell-specific expression, the antibody can be tested using ovarian or testicular tissue lysates or sections. Given the restricted expression, appropriate negative controls should be included.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive one-stop sourcing solution for IVD raw materials, including validated antibody pairs, monoclonal/polyclonal antibodies, and ancillary reagents. This DDX4 (VASA) monoclonal antibody, targeting an essential germ cell marker, supports research and diagnostic development in reproductive biology and oncology. Our supply capabilities extend to rare target raw-material sourcing, ensuring seamless transition from concept to clinic.