Anti-DDX4 Rabbit Polyclonal Antibody for WB, IF-P, IHC-P, ELISA - Q9NQI0

Core Product Specifications and Parameters

Biological Background: DDX4 Function and Localization

• DDX4, also known as VASA, is a member of the DEAD-box RNA helicase family and functions as a probable ATP-dependent RNA helicase.
• It is essential for spermatogenesis, playing a critical role in germ cell development and differentiation (PubMed:10920202, PubMed:21034600).
  Related references: PMID:10920202, PMID:21034600
• DDX4 is involved in transposon silencing via the piRNA pathway, helping to maintain genomic integrity in germ cells (By similarity).
• It participates in secondary piRNA biogenesis, including PIWIL2-triggered piRNA production, utilizing its helicase activity for processing pre-piRNAs (By similarity).
• The protein localizes to the cytoplasm and perinuclear region.
• Expression is highly tissue-specific, found only in ovary and testis, and in migratory primordial germ cells.
• DDX4 is regulated by phosphorylation and subject to alternative splicing.
• Belongs to a broad range of biological processes including developmental protein, differentiation, meiosis, and RNA-mediated gene silencing.

Experimental Guidance and Technical Tips

• The antibody was generated against a recombinant fragment covering amino acids 635–724 at the C-terminus of human DDX4. This region is likely conserved and may detect multiple isoforms.
• For Western blot, a band at approximately 79 kDa is expected. Validate with appropriate positive controls from testis or ovary lysates, using relevant species (human, mouse, rat).
• For immunofluorescence (IF-P) and immunohistochemistry (IHC-P), expect cytoplasmic and perinuclear staining. Use appropriate fixation and permeabilization, and consider antigen retrieval for IHC-P.
• Cross-reactivity with mouse and rat was demonstrated, enabling use in these model organisms. Optimize antibody dilutions and conditions for each application and species.
• Due to the restricted expression in germ cells, ensure tissue specificity during assay development.
• This polyclonal antibody may be suitable for ELISA-based detection, but performance should be validated in the desired sandwich or indirect format.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering reliable antibodies like anti-DDX4 for reproductive health and germ cell research. We provide validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets, supporting diagnostic manufacturers and laboratories from concept to clinic. Contact CamelBio to explore how our DDX4 antibody and associated reagents can accelerate your assay development.