Anti-DDX3Y Rabbit Polyclonal Antibody for WB, ELISA - O15523

Core Product Specifications and Parameters

Biological Background: DDX3Y Function and Localization

• DDX3Y is an ATP-dependent RNA helicase belonging to the DEAD-box protein family, encoded by the Y-chromosomal gene DDX3Y (alias DBY).
• The protein functions putatively to unwind RNA secondary structures, and during immune response, it may enhance IFNB1 expression via the IRF3/IRF7 pathway.
• DDX3Y is widely expressed at the mRNA level, with highest levels in testis; at the protein level, it is testis-specific and predominantly expressed in spermatogonia. Related references: PMID:9381176 and PMID:15294876
• Subcellular localization includes both cytoplasm and nucleus, consistent with roles in RNA metabolism and gene regulation.
• The protein contains conserved domains for ATP binding, RNA binding, and helicase activity, and is subject to post-translational modifications including phosphorylation, acetylation, methylation, and ubiquitination.
• DDX3Y has been identified in proteomics studies, underscoring its expression and potential roles in various cellular processes.
• The Y-chromosomal location implicates DDX3Y in male-specific functions, particularly in spermatogenesis.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fragment corresponding to the N-terminal region (amino acids 1-210), suggesting the antibody may recognize epitopes within this domain; consider this when designing constructs or truncations.
• For Western blot (WB), due to the testis-specific protein expression, use lysates from human or rat testis as positive controls; validate in cell lines with known expression if possible.
• The predicted molecular weight is 73 kDa; monitor for band shifts that may reflect post-translational modifications such as phosphorylation or acetylation.
• For ELISA, the recombinant immunogen or purified protein may serve as a suitable coating antigen; optimize coating concentration and antibody dilution experimentally.
• As a polyclonal antibody, lot-to-lot consistency should be verified in the intended assay; consider including a reference standard if quantitative results are required.
• Cross-reactivity with rat is indicated; in rat samples, validate specificity using appropriate controls, as epitope conservation may vary.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for diagnostic manufacturers, laboratories, and research institutes, offering a seamless supply chain from concept to clinic. This anti-DDX3Y polyclonal antibody is applicable to reproductive biology, immunology, and oncology research. Beyond individual antibodies, CamelBio provides validated antibody pairs, optimized monoclonal/polyclonal preparations, bulk ancillary reagents, and can assist in sourcing rare target raw materials to support your IVD development programs.