Anti-DDX3Y Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - O15523

Core Product Specifications and Parameters

Biological Background: DDX3Y Function and Localization

• DDX3Y, also known as DBY, is a member of the DEAD box protein family, specifically DEAD box protein 3, Y-chromosomal.
• It functions as a probable ATP-dependent RNA helicase, playing a role in unwinding RNA secondary structures in an ATP-dependent manner.
• During immune responses, DDX3Y may enhance IFNB1 expression via the IRF3/IRF7 signaling pathway (By similarity).
• Subcellular localization includes both cytoplasm and nucleus.
• DDX3Y undergoes several post-translational modifications: acetylation, phosphorylation, methylation, and is subject to ubiquitin-like conjugation.
• The protein is primarily expressed at the protein level in testis, predominantly in spermatogonia, though occasionally detected in some pre-leptotene/leptotene spermatocytes (PubMed:9381176, PubMed:15294876). Related references: PMID:9381176 PMID:15294876

Experimental Guidance and Technical Tips

• The immunogen (amino acids 1-210) corresponds to the N-terminal region of DDX3Y, which may allow detection of total protein irrespective of post-translational modifications; consider using this against full-length or truncated forms.
• For Western blot, a band at approximately 73 kDa is expected; optimize blocking and antibody dilution for your sample type, and include positive controls like testis lysate.
• In immunohistochemistry (paraffin), testis tissue serves as a suitable positive control due to high DDX3Y expression; antigen retrieval conditions (e.g., citrate buffer pH 6.0) may need adjustment.
• For immunofluorescence/immunocytochemistry, fix and permeabilize cells appropriately; confirm localization in both cytoplasm and nucleus.
• In ELISA applications, the polyclonal nature may provide good sensitivity; pair with a monoclonal antibody for sandwich ELISA design if needed.
• Cross-reactivity with mouse and rat DDX3Y is indicated, enabling translational studies in rodent models; however, validate specificity in the relevant species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material procurement for diagnostic developers and researchers. For DDX3Y-related studies — spanning reproductive biology and innate immunity — we provide high-quality polyclonal and monoclonal antibodies, complementary antigen pairs, and bulk ancillary reagents to accelerate assay development. Our sourcing expertise also covers rare targets and custom immunogen design to meet your project needs.