Anti-DDX3X Polyclonal Antibody for WB, IHC-P, IF/ICC, IP, ELISA - O00571

Core Product Specifications and Parameters

Biological Background: DDX3X Function and Localization

• ATP-dependent RNA helicase with relaxed substrate specificity; can unwind DNA/RNA duplexes and bind RNA G-quadruplex structures (e.g., in NRAS mRNA). Related references: PMID:17357160, PMID:21589879, PMID:29222110, PMID:30256975

• Regulates transcription: positively regulates CDKN1A/WAF1/CIP1 via SP1, represses CDH1/E-cadherin, and potentiates HNF4A-mediated MTTP expression; also may regulate TP53. These functions often require ATPase but not helicase activity. Related references: PMID:16818630, PMID:18264132, PMID:28128295, PMID:28842590

• Involved in translation: promotes translation of mRNAs with structured 5'-UTRs (e.g., cyclin E1/CCNE1) via helicase activity, and enhances IRES-mediated translation independently of helicase. Also facilitates 80S ribosome assembly. Related references: PMID:20837705, PMID:22872150, PMID:22323517, PMID:17667941

• Key player in innate immunity: acts as a viral RNA sensor, promoting type I interferon production via MAVS/RIG-I and TLR7/TLR8 pathways, and interacts with TBK1/IKBKE to activate IRF3/IRF7. Related references: PMID:20127681, PMID:21170385, PMID:23478265, PMID:30341167

• Modulates stress responses: required for stress granule assembly (pro-survival) via EIF4E interaction, and also regulates NLRP3 inflammasome assembly (pyroptosis), acting as a checkpoint.

• Subcellular localization: found in cytoplasm, nucleus, stress granules, inflammasomes, cell membrane, lamellipodia, and centrosome.

• Tissue specificity: widely expressed; in testis, expressed in spermatids; also in epidermis and liver at protein level. Related references: PMID:15294876, PMID:16301996, PMID:16818630

• Post-translational modifications and keywords: acetylation, phosphorylation, methylation, ubiquitination; involved in apoptosis, cell cycle, chromosome segregation, and host-virus interactions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-100) of human DDX3X; for WB, consider using whole-cell or tissue lysates and confirm reactivity with appropriate controls (predicted MW ~73 kDa).
• For IHC-P and IF/ICC, validate fixation and permeabilization conditions; DDX3X localizes to both cytoplasm and nucleus.
• This polyclonal antibody is suitable for IP and ELISA; optimize antibody concentration for each specific assay.

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CamelBio serves as a one-stop sourcing partner for diagnostic manufacturers and research laboratories, providing validated antibodies and raw materials for targets like DDX3X. Our portfolio includes polyclonal and monoclonal antibodies, bulk reagents, and rare-target sourcing to support assay development and production. From immunology to oncology research, CamelBio streamlines your supply chain from concept to clinic.