Anti-DDX24 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q9GZR7

Core Product Specifications and Parameters

Biological Background: DDX24 Function and Localization

• DDX24 (DEAD box protein 24) is an ATP-dependent RNA helicase encoded by the DDX24 gene in humans.
• It plays a central role in pre-mRNA splicing and influences cell cycle regulation and innate immunity. Related references: PMID:24204270 PMID:24980433
• DDX24 negatively regulates TP53 transcriptional activity by inhibiting its EP300-mediated acetylation, thereby controlling cell growth arrest and senescence. Related references: PMID:25867071
• It exhibits antiviral activity by recognizing gammaherpesvirus transcripts during lytic reactivation. Related references: PMID:36298642
• The protein localizes to both the cytoplasm and nucleus, shuttling between compartments to perform its diverse functions.
• DDX24 is ubiquitously expressed, with highest levels in heart and brain, and lowest in thymus and small intestine.
• Post-translational modifications include acetylation, phosphorylation, and ubiquitination, reflecting its multi-level regulation.
• In the context of microbial infection, DDX24 facilitates HIV-1 replication by enhancing Rev-dependent nuclear export of viral RNAs. Related references: PMID:24204270

Experimental Guidance and Technical Tips

• The immunogen spans the C-terminal region (aa 510-859) of human DDX24, which may confer specificity for this helicase in immunological assays.
• For Western blot (WB), the estimated molecular weight is ~96 kDa; verify band size using appropriate molecular weight markers and positive control lysates from human, mouse, or rat tissues.
• In immunofluorescence/ICC, consider nuclear and cytoplasmic staining patterns; fixation and permeabilization conditions should be optimized for the specific cell type.
• ELISA performance may depend on coating antigen and detection system; pilot experiments are recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated partner for IVD raw material procurement, offering a seamless bridge from concept to clinic. This anti-DDX24 polyclonal antibody exemplifies our commitment to providing high-quality reagents for RNA biology and infectious disease research. We supply a comprehensive range of diagnostic antibodies, antigens, and ancillary reagents, backed by technical consultation to accelerate your assay development.