Anti-DDX20 Polyclonal Antibody for WB, IF/ICC, ELISA - Q9UHI6

Core Product Specifications and Parameters

Biological Background: DDX20 Function and Localization

• Protein identity and isoforms: Probable ATP-dependent RNA helicase DDX20 (also known as Gemin3, DEAD box protein 20, DP103) is encoded by the DDX20 gene; alternative splicing yields multiple isoforms.
• Core snRNP assembly: DDX20 is a component of the SMN complex that catalyzes the assembly of small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome, thereby playing an important role in pre-mRNA splicing.
• Sm protein ring assembly: In the cytosol, the SMN complex accepts trapped Sm proteins from the chaperone CLNS1A and, upon snRNA binding, evicts to allow complete assembly of the heptameric Sm core ring.
• SnoRNP metabolism: May also play a role in the metabolism of small nucleolar ribonucleoproteins (snoRNPs).
• Subcellular localization: Localizes to the cytoplasm, nucleus, and gems (gemini of coiled bodies).
• Post-translational modifications and keywords: Classified as a phosphoprotein; features ATP-binding, DNA-binding, helicase, hydrolase, and nucleotide-binding domains; involved in mRNA processing and splicing.
• Tissue specificity: Ubiquitous expression across tissues.
• Gene family: Belongs to the DEAD-box helicase family.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 25-280 of human DDX20 (NP_009135.4), an N-terminal region that may confer specificity. Consider validating antibody reactivity in your target species and sample type.
• Recommended applications include Western blot (WB), immunofluorescence/immunocytochemistry (IF/ICC), and ELISA. When using WB, note the predicted molecular weight is ~92 kDa.
• Since cross-reactivity is reported for human, mouse, and rat, the antibody may be suitable for studies in these species. However, optimization of dilution and conditions is advised.
• For IF/ICC, fixation/permeabilization methods may affect epitope accessibility; test different protocols.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated IVD raw-material partner, CamelBio supports diagnostic manufacturers and researchers with a comprehensive range of antibodies, antigens, and ancillary reagents. This product targets DDX20, a key player in snRNP assembly and splicing, and is sourced to facilitate your research and assay development needs. We offer validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk reagents, and rare-target raw-material sourcing—streamlining your path from concept to clinic.