Anti-DDX1 Polyclonal Antibody - Q92499

Core Product Specifications and Parameters

Biological Background: DDX1 Function and Localization

• DDX1 is an ATP-dependent RNA helicase, also known as DEAD box protein 1 or DEAD box protein retinoblastoma (DBP-RB).
• Acts as an ATP-dependent RNA helicase that unwinds RNA-RNA and RNA-DNA duplexes; possesses 5' single-stranded RNA overhang nuclease activity and ATPase activity on various RNA polynucleotides.
• May play a role in RNA clearance at DNA double-strand breaks (DSBs), facilitating template-guided repair of transcriptionally active genomic regions.
• Together with RELA, acts as a coactivator to enhance NF-kappa-B-mediated transcriptional activation; positively regulates CCND2 expression by binding to its promoter.
• May be involved in 3'-end cleavage and polyadenylation of pre-mRNAs; is an accessory subunit of the tRNA-splicing ligase complex that joins spliced tRNA halves. This step requires cooperation with ZBTB8OS for guanylylation of RTCB. Related references: PMID:24870230
• Component of a multi-helicase-TICAM1 complex that acts as a cytoplasmic sensor of viral dsRNA, activating antiviral responses including pro-inflammatory cytokine induction. Binds poly(I:C) dsRNA.
• Found in the nucleus, cytoplasm, cytoplasmic granules, cytosol, and mitochondria.
• Highest expression in retinoblastoma cell lines and neuroectodermal tissues (retina, brain, spinal cord).

Experimental Guidance and Technical Tips

• Validate antibody specificity and titer in the relevant sample system using positive and negative controls, especially for applications like IHC-P and IF/ICC.
• For WB, consider using reducing denaturing conditions given the predicted molecular weight of approximately 82kDa.
• For IHC-P, optimize antigen retrieval methods and antibody dilution; for IF/ICC, carefully evaluate fixation and permeabilization conditions.
• Cross-reactivity with mouse and rat extends utility to these species, but independent validation is recommended.
• IP and ELISA applications may require optimization of antibody concentration and buffer conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with streamlined access to premium IVD raw materials, including validated antibody pairs and optimized polyclonal antibodies like this anti-DDX1 pAb. Our comprehensive supply capabilities cover bulk ancillary reagents and rare target sourcing, supporting studies on RNA helicases, transcriptional regulation, and antiviral immunity. From concept to clinic, CamelBio offers the expertise and reliable supply channels needed to accelerate your development pipeline.