Anti-DDR2 Rabbit Monoclonal Antibody for WB and ELISA - Q16832

Core Product Specifications and Parameters

Biological Background: DDR2 Function and Localization

• DDR2 (Discoidin domain-containing receptor 2) is a receptor tyrosine kinase also known as CD167b, NTRKR3, TKT, and TYRO10.
• It functions as a cell surface receptor for fibrillar collagen, regulating tissue remodeling through the activation of downstream signaling pathways. Related references: PMID:30449416
• DDR2 plays a critical role in bone development by regulating osteoblast differentiation and chondrocyte maturation via MAP kinase signaling and RUNX2 activation.
• It promotes extracellular matrix remodeling by upregulating collagenases MMP1, MMP2, and MMP13, thereby facilitating cell migration and tumor invasion.
• DDR2 contributes to wound healing by promoting fibroblast migration and proliferation.
• Subcellular localization: primarily localized to the cell membrane as a transmembrane receptor.
• Tissue expression: detected at high levels in heart and lung, with lower expression in brain, placenta, liver, skeletal muscle, pancreas, and kidney (at protein level).
• Post-translational modifications include tyrosine autophosphorylation upon collagen binding, and glycosylation (evidenced by keywords: phosphoprotein, glycoprotein).

Experimental Guidance and Technical Tips

• Based on the immunogen design (amino acids 756-855, intracellular domain), this antibody is expected to recognize the C-terminal region of DDR2. For Western blot, look for a band near 97 kDa in lysates from DDR2-expressing tissues (e.g., heart, lung).
• Consider validating the antibody’s reactivity in your specific sample system; collagen stimulation may enhance DDR2 expression and phosphorylation levels for downstream detection.
• For ELISA applications, optimize coating and detection conditions using the immunogen peptide as a positive control.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutions with streamlined access to premium IVD raw materials, including validated antibodies for targets such as DDR2. Our platform offers a comprehensive range of antibody pairs, monoclonal/polyclonal antibodies, and bulk ancillary reagents to support assay development. Through strategic supplier partnerships, we also facilitate sourcing of rare-target raw materials, accelerating your project from concept to clinic.