Anti-DDIT3/CHOP Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P35638

Core Product Specifications and Parameters

Biological Background: DDIT3/CHOP Function and Localization

• DDIT3/CHOP is a multifunctional transcription factor that plays an essential role in endoplasmic reticulum (ER) stress response, inducing cell cycle arrest and apoptosis under stress conditions. Related references: PMID:15322075, PMID:15775988, PMID:19672300
• Acts as a dominant-negative inhibitor of CCAAT/enhancer-binding protein (C/EBP) transcription, dimerizing with C/EBP family members and impairing their DNA-binding activity.
• Positively regulates transcription of pro-apoptotic and inflammatory genes, including TRIB3, IL6, IL8, BBC3/PUMA, and BCL2L11/BIM. Related references: PMID:15775988, PMID:17709599, PMID:20876114
• Negatively regulates anti-apoptotic BCL2, differentiation factor MYOD1, and ATF4-dependent ASNS transcription, promoting cell death. Related references: PMID:18940792, PMID:19672300, PMID:20829347
• Inhibits canonical Wnt signaling by directly binding TCF7L2/TCF4 and repressing its transcriptional activity. Related references: PMID:16434966
• Localizes to both cytoplasm and nucleus; undergoes alternative initiation and splicing, and is classified as a phosphoprotein involved in apoptosis, cell cycle arrest, and the unfolded protein response.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N‑terminal region (aa 1‑169), so epitope mapping may be focused on this domain.
• In WB, expect a band near 19kDa; validation with ER‑stress‑induced cell lysates (e.g., tunicamycin‑treated) is recommended.
• For IHC‑P and IF/ICC, optimize antigen retrieval protocols to detect nuclear translocation.
• Cross‑reactivity with mouse and rat enables preclinical translational studies; verify signaling consistency across species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with a one‑stop source for IVD raw materials, including validated antibodies, antigen pairs, and rare‑target reagents. This DDIT3/CHOP polyclonal antibody supports ER stress, apoptosis, and unfolded protein response research, and fits seamlessly into larger procurement plans for related biomarker panels or assay development. Contact us for bulk orders or custom sourcing solutions.