Anti-DDIT3/CHOP Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P35638

Core Product Specifications and Parameters

Biological Background: DDIT3/CHOP Function and Localization

• DDIT3, also known as CHOP, is a DNA damage-inducible transcript 3 protein and a member of the C/EBP family of transcription factors, functioning as a dominant-negative regulator and a stress-induced activator.
• It plays a central role in the ER stress response, inducing cell cycle arrest and apoptosis upon unresolved ER stress. Related references: PMID:15322075, PMID:15775988, PMID:19672300
• As a transcription factor, DDIT3 positively regulates pro-apoptotic and stress-related genes including TRIB3, BBC3/PUMA, and BCL2L11/BIM, while negatively regulating anti-apoptotic BCL2 and cell differentiation factors like MYOD1. Related references: PMID:15775988, PMID:17709599, PMID:20876114, PMID:18940792, PMID:19672300, PMID:20829347
• It inhibits the canonical Wnt signaling pathway by binding to TCF7L2/TCF4, impairing its DNA-binding and transcriptional activity. Related references: PMID:16434966
• DDIT3 localizes to both the cytoplasm and nucleus, consistent with its roles in signal transduction and gene regulation.
• Post-translational modifications include phosphorylation and ubiquitin-like conjugation, which may modulate its activity and stability.
• It is implicated in chromosomal rearrangements associated with myxoid liposarcoma, where a fusion with FUS results in an oncogenic transcription factor.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-100) of human DDIT3; consider using the immunizing peptide as a blocking control in relevant assays.
• For Western blotting, a band of approximately 19 kDa is expected; verify expression in ER stress-induced samples.
• For IHC-P and IF/ICC, optimize antigen retrieval and fixation conditions according to the sample type.
• Cross-reactivity with mouse and rat is reported; validate in the appropriate species model systems.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for diagnostic manufacturers and laboratories, providing validated antibody pairs, high-quality monoclonal antibodies, and bulk ancillary reagents. This DDIT3/CHOP antibody supports research into ER stress and apoptosis pathways. For customized or rare-target raw material sourcing, contact CamelBio's experts.