Anti-DDIT3/CHOP Monoclonal Antibody for WB, IHC-P, ELISA - P35638

Core Product Specifications and Parameters

Biological Background: DDIT3/CHOP Function and Localization

• DNA damage-inducible transcript 3 (DDIT3), also known as CHOP, C/EBP zeta, and GADD153, is a multifunctional transcription factor that belongs to the C/EBP family.
• Plays an essential role in the endoplasmic reticulum (ER) stress response, inducing cell cycle arrest and apoptosis under stress conditions. Related references: PMID:15322075, PMID:15775988
• Acts as a dominant-negative regulator of C/EBP-induced transcription by forming dimers with C/EBP family members, preventing their DNA binding and inhibiting target gene expression.
• Positively regulates the transcription of pro-apoptotic and inflammatory genes, including TRIB3, TNFRSF10B/DR5, BBC3/PUMA, BCL2L11/BIM, IL6, IL8, and IL23. Related references: PMID:15775988, PMID:17709599, PMID:20876114
• Negatively regulates the expression of anti-apoptotic BCL2 and differentiation factor MYOD1, and inhibits ATF4- and CEBPA-dependent transcriptional activation. Related references: PMID:18940792, PMID:19672300, PMID:20829347
• Localizes to both the cytoplasm and nucleus, consistent with its dual role in cytoplasmic stress signaling and nuclear gene regulation.
• Post-translationally modified by phosphorylation and ubiquitin conjugation, which may regulate its stability and activity.
• Additionally inhibits the canonical Wnt signaling pathway by binding to TCF7L2/TCF4 and repressing its transcriptional activity. Related references: PMID:16434966

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1-100) of human DDIT3; therefore, the antibody likely recognizes an epitope within this domain.
• For Western blotting, a band near 19 kDa is expected, but consider that post-translational modifications may cause slight shifts in apparent molecular weight.
• In immunohistochemistry (paraffin-embedded tissues), optimize antigen retrieval and validate staining in tissues with known ER stress or apoptosis induction, such as treated cancer cell lines or disease models.
• For ELISA, ensure the coating peptide or protein is within the N-terminal 1-100 region to capture the antibody effectively; cross-reactivity with rodent orthologs may be leveraged for multi-species assays.
• As with any antibody, performance should be validated in the end-user's specific sample system and experimental conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio specializes in providing diagnostic manufacturers, laboratories, and research institutions with comprehensive access to IVD raw materials. For studies on ER stress, apoptosis, and transcription factor regulation, we supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. Our sourcing network also supports rare-target material procurement, ensuring a seamless supply chain from concept to clinic.