Anti-DDC Polyclonal Antibody for WB, IF-P, IHC-P, ELISA - P20711

Core Product Specifications and Parameters

Biological Background: Aromatic-L-amino-acid decarboxylase (DDC) Function and Localization

• Aromatic-L-amino-acid decarboxylase (DDC), also known as DOPA decarboxylase, is encoded by the DDC gene in humans.
• Catalyzes the decarboxylation of L-3,4-dihydroxyphenylalanine (DOPA) to dopamine and L-5-hydroxytryptophan to serotonin, a key step in catecholamine and serotonin biosynthesis.
• Functions as a pyridoxal phosphate (PLP)-dependent lyase belonging to the decarboxylase family.
• Highly expressed in the kidney, with additional roles in neurotransmitter production in neural and neuroendocrine tissues.
• Undergoes post-translational acetylation, and alternative splicing generates multiple isoforms.
• Mutations in DDC are associated with aromatic L-amino acid decarboxylase deficiency, a rare neurometabolic disorder.
• Identified as part of the reference proteome and in multiple proteomics studies, underscoring its biological significance.

Experimental Guidance and Technical Tips

• For Western blotting, the antibody detects a band at approximately 54 kDa; validate against appropriate positive controls and consider loading controls.
• In immunofluorescence and immunohistochemistry, optimize fixation and permeabilization protocols for the target tissue; test a range of antibody dilutions.
• For ELISA applications, evaluate the antibody as a capture or detection reagent using recombinant DDC protein as standard.
• Given the validated cross-reactivity with mouse and rat, this antibody may be used for studies in these species; however, verify reactivity in your specific experimental context.
• Consider the immunogen region (N-terminal amino acids 1-137) when interpreting results, as certain isoforms or cleavage products may not be detected.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-DDC polyclonal antibody supports research into neurotransmitter metabolism, catecholamine biosynthesis, and related neurological disorders. Beyond this product, CamelBio can supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and assist in sourcing rare-target raw materials to streamline your IVD development.