Anti-DDB2 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q92466

Core Product Specifications and Parameters

Biological Background: DDB2 Function and Localization

• DDB2 (DNA damage-binding protein 2) is the substrate recognition subunit of the UV-DDB complex, which senses UV-induced DNA lesions including cyclobutane pyrimidine dimers (CPD), 6-4 photoproducts (6-4 PP), apurinic sites, and short mismatches. Related references: PMID:10882109, PMID:11278856, PMID:11705987

• As part of the DCX (DDB1-CUL4-X-box) E3 ubiquitin ligase complex DDB2-CUL4-ROC1, DDB2 directs ubiquitination of histone H2A, H3, and H4 at damage sites, potentially facilitating their removal from nucleosomes to promote repair. Related references: PMID:12732143, PMID:16473935, PMID:16678110

• DDB2-containing E3 ligase also ubiquitinates XPC protein to enhance DNA binding and NER, and targets KAT7/HBO1 for degradation upon ATR-mediated phosphorylation in the DNA damage response. Related references: PMID:15882621, PMID:26572825

• DDB2 localizes predominantly to the nucleus and is associated with chromatin, reflecting its direct involvement in genomic surveillance and DNA repair processes.

• Ubiquitously expressed, DDB2 shows highest levels in corneal endothelium, with multiple alternatively spliced isoforms exhibiting tissue-specific distribution patterns.

• DDB2 is classified under keywords including DNA repair, DNA damage, and Ubl conjugation pathway; mutations in DDB2 are linked to the skin cancer-prone disorder xeroderma pigmentosum (XP) group E.

Experimental Guidance and Technical Tips

• The immunogen spans the N-terminal region (aa 1-315) and may detect multiple DDB2 isoforms; verify isoform specificity by comparing band sizes in western blot.
• For WB, a single band of ~48 kDa is expected; include UV-treated positive controls to monitor damage-dependent post-translational modifications.
• In IF/ICC applications, nuclear staining should be apparent; co-localization with DNA damage markers (e.g., γH2AX) can confirm recruitment to lesion sites.
• For ELISA, use the recombinant immunogen as a standard and assess cross-reactivity with lysates from mouse and rat, given the confirmed species cross-reactivity.
• Due to the involvement of DDB2 in ubiquitination pathways, consider using proteasome inhibitors or deubiquitinase inhibitors to stabilize ubiquitinated forms in certain experimental setups.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines access to high-quality IVD raw materials, from validated antibody pairs to optimized polyclonal antibodies like this DDB2 pAb. For DNA damage and repair research, we also provide bulk ancillary reagents and rare target sourcing to support your assay development from concept to clinic.