Anti-DDB2 Rabbit Monoclonal Antibody for WB, ELISA - Q92466

Core Product Specifications and Parameters

Biological Background: DDB2 Function and Localization

• DDB2 (DNA damage-binding protein 2) is a core component of the UV-DDB complex, which recognizes UV-induced DNA lesions such as cyclobutane pyrimidine dimers and 6-4 photoproducts, thereby recruiting nucleotide excision repair (NER) machinery. Related references: PMID:10882109, PMID:11278856, PMID:11705987
• It also serves as the substrate recognition module for the DDB2-CUL4-ROC1 E3 ubiquitin ligase complex, mediating ubiquitination of histones H2A, H3, and H4 at damage sites to facilitate chromatin remodeling and repair. Related references: PMID:12732143, PMID:16473935, PMID:16678110
• This complex additionally ubiquitinates XPC, enhancing its DNA-binding and promoting NER, and targets KAT7/HBO1 for degradation upon DNA damage. Related references: PMID:15882621, PMID:26572825
• DDB2 localizes predominantly to the nucleus and chromosomes, consistent with its role in DNA repair.
• The protein contains WD40 repeat domains and undergoes post-translational modifications including phosphorylation and ubiquitination.
• Alternative splicing yields multiple isoforms; mutations in DDB2 are associated with xeroderma pigmentosum complementation group E.
• DDB2 is ubiquitously expressed, with highest levels in corneal endothelium; isoform D1 is particularly abundant in brain and heart.

Experimental Guidance and Technical Tips

• Given that the immunogen spans amino acids 220–427 (the C-terminal region), confirm that your target isoform(s) of interest contain this sequence to ensure reactivity.
• For Western blot, nuclear-enriched lysates from UV-treated human cells may provide optimal signal due to DDB2’s nuclear localization and damage-induced recruitment.
• In ELISA applications, test both direct and sandwich formats; pairing with another DDB2 antibody may enhance specificity.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated IVD raw-material partner, CamelBio provides a comprehensive portfolio spanning validated antibody pairs, bulk reagents, and rare-target sourcing to accelerate diagnostic development. This anti-DDB2 antibody supports research into DNA damage response and repair pathways, and we offer tailored solutions for assay design and scaling. From concept to clinic, CamelBio is your bridge to reliable, high-quality immunoassay components.