Anti-DDB1 Rabbit Polyclonal Antibody for WB, IHC-P, IP, ELISA - Q16531

Core Product Specifications and Parameters

Biological Background: DDB1 Function and Localization

• DDB1 (DNA damage-binding protein 1), also known as XPE, DDBA, XAP1, and UV-DDB1, is a 127 kDa protein that serves as a core component of the UV-DDB complex, which recognizes UV-induced DNA lesions such as cyclobutane pyrimidine dimers (CPD) and 6-4 photoproducts to initiate nucleotide excision repair (NER).
  Related references: PMID:15448697, PMID:16260596, PMID:16407242

• DDB1 is a substrate adaptor for multiple DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complexes, which mediate ubiquitination and subsequent proteasomal degradation of target proteins, with functional specificity dictated by the variable substrate recognition component recruited.
  Related references: PMID:14739464, PMID:16407252, PMID:16482215

• Through DCX(DDB2) complex, DDB1 facilitates the ubiquitination of histones H2A, H3, and H4 at UV-damaged sites, promoting histone removal from nucleosomes to facilitate DNA repair.
  Related references: PMID:16473935, PMID:16678110, PMID:17041588

• DDB1 is also involved in the ubiquitination of XPC, enhancing its DNA-binding activity and promoting NER, and participates in transcription-coupled repair (TCR) via the DCX(ERCC8) complex.
  Related references: PMID:15882621, PMID:12732143, PMID:32355176, PMID:38316879

• The DDB1-CUL4A-DTL E3 ligase complex regulates circadian clock function by mediating the ubiquitination and degradation of CRY1, and influences gluconeogenesis through FOXO1 stabilization.
  Related references: PMID:26431207

• Subcellular localization includes both cytoplasm and nucleus, reflecting its roles in DNA repair signaling and protein degradation pathways.

• DDB1 is subject to post-translational modifications including acetylation, phosphorylation, and ubiquitination, and is implicated in viral interactions and intellectual disability-associated variants.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1000-1140 in the C-terminal region of human DDB1. For western blotting (WB), a band at approximately 127 kDa is expected; consider the possibility of detecting splice isoforms if the epitope is conserved.
• For immunohistochemistry on paraffin sections (IHC-P), antigen retrieval conditions may need optimization due to the large protein size and potential nuclear localization.
• When performing immunoprecipitation (IP), antibody concentration and incubation time should be empirically determined, as polyclonal preparations may vary between lots.
• In ELISA, the antibody can be used as a capture or detection reagent; validate cross-reactivity against mouse and rat DDB1 if working with multispecies samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and research laboratories with one-stop access to high-quality IVD raw materials. This anti-DDB1 antibody supports DNA damage repair and ubiquitination studies, and we can supply validated antibody pairs, bulk monoclonal and polyclonal reagents, and ancillary materials for your assay development. Whether you need rare target sourcing or optimized reagents for your pipeline, CamelBio bridges the gap from concept to clinic.