Anti-DDB1 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - Q16531

Core Product Specifications and Parameters

Biological Background: DDB1 Function and Localization

• DDB1 (DNA damage-binding protein 1), also known as DDB p127 or XAP-1, is a core component of the UV-DDB complex that recognizes UV-induced DNA damage (CPD, 6-4 PP) and recruits nucleotide excision repair (NER) pathway proteins to initiate repair. Related references: PMID:15448697, PMID:16260596, PMID:16407242
• DDB1 functions as a substrate receptor for DCX (DDB1-CUL4-X-box) E3 ubiquitin ligase complexes, determining specificity for target protein ubiquitination and subsequent proteasomal degradation. Related references: PMID:14739464, PMID:16407252, PMID:16482215
• The DCX(DDB2) complex ubiquitinates histones H2A, H3, and H4 at UV-damaged sites, facilitating nucleosome remodeling and promoting DNA repair. Related references: PMID:16473935, PMID:16678110, PMID:17041588
• DDB1 also mediates ubiquitination of the repair factor XPC via DCX(DDB2), enhancing XPC DNA-binding ability and promoting NER. Related references: PMID:15882621
• In addition, DCX(DTL) mediates ubiquitination of CDT1 and TP53 in response to DNA damage and replication stress. Related references: PMID:17041588
• Beyond DNA repair, DDB1 regulates circadian rhythm by mediating CRY1 ubiquitination and degradation, and contributes to liver gluconeogenesis via FOXO1 stabilization. Related references: PMID:26431207
• DDB1 is localized in both the cytoplasm and nucleus, consistent with its roles in DNA repair and protein degradation.
• The protein undergoes multiple post-translational modifications including acetylation and phosphorylation, and is linked to disease variants, host-virus interactions, and alternative splicing.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 1041-1140 of human DDB1. When assessing specificity, consider whether your detection target covers this C-terminal region.
• For Western blotting (WB), the predicted molecular weight is approximately 127 kDa. Validate the observed band size in your specific sample system and consider using appropriate controls.
• This antibody is suitable for immunohistochemistry (IHC-P). Optimization of antigen retrieval conditions and antibody dilution is recommended for your tissue type.
• ELISA can be utilized for titer determination or quantitative assays; indirect ELISA format may be considered.
• Cross-reactivity with mouse and rat DDB1 is indicated, but always confirm performance in your species of interest.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and research laboratories with a comprehensive one-stop supply of IVD raw materials, including validated antibody pairs and monoclonal antibodies like this DDB1 antibody. From bulk ancillary reagents to sourcing rare targets, we streamline your workflow from concept to clinic. Explore our catalog for essential DNA repair and ubiquitination research tools.