Anti-DDB1 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q16531

Core Product Specifications and Parameters

Biological Background: DDB1 Function and Localization

• DDB1 is a multifunctional protein involved in DNA repair as part of the UV-DDB complex and in protein ubiquitination as a component of DCX (DDB1-CUL4-X-box) E3 ubiquitin ligase complexes. Related references: PMID:14739464 PMID:15448697 PMID:16260596
• As a core component of the UV-DDB complex, it recognizes UV-induced DNA damage (cyclobutane pyrimidine dimers, 6-4 photoproducts, apurinic sites) and recruits nucleotide excision repair (NER) factors to initiate repair. Related references: PMID:15448697 PMID:16260596 PMID:16407242
• DDB1 serves as the adaptor for multiple DCX E3 ligases; substrate specificity is determined by the associated DCAF (DDB1- and CUL4-associated factor). Related references: PMID:14739464 PMID:16407252 PMID:16482215
• The DCX(DDB2) complex ubiquitinates histones H2A, H3, H4 at UV damage sites, facilitating their removal and promoting repair, and also ubiquitinates XPC to enhance DNA binding. Related references: PMID:16473935 PMID:16678110 PMID:17041588 PMID:15882621
• DCX(DTL) mediates polyubiquitination of CDT1 and TP53, linking DDB1 to DNA replication and damage responses. Related references: PMID:17041588
• DCX(ERCC8) (the CSA complex) functions in transcription-coupled repair (TCR). Related references: PMID:12732143 PMID:32355176 PMID:38316879
• DDB1 regulates the circadian clock by mediating CRY1 degradation via the CUL4A-DTL E3 ligase, and influences hepatic gluconeogenesis through FOXO1. Related references: PMID:26431207
• Localizes to both cytoplasm and nucleus; undergoes acetylation, phosphorylation, and alternative splicing; associated with disease variants and host-virus interactions.

Experimental Guidance and Technical Tips

• For western blot, expect a major band at approximately 127 kDa; validate using whole cell lysates from human, mouse, or rat cell lines.
• For IHC-P, consider heat-induced antigen retrieval and test on tissues known to express DDB1 (e.g., skin, liver).
• For IF/ICC, optimize fixation and permeabilization conditions as the detection of nuclear and cytoplasmic signals may vary.
• The immunogen maps to the C-terminal half of DDB1; thus the antibody should recognize all full-length isoforms but may not detect N-terminal truncations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials and technical support for diagnostic development. This anti-DDB1 polyclonal antibody targets a key player in DNA repair and ubiquitination pathways, making it a useful tool for cancer, genomic stability, and dermatological research. Beyond antibodies, we supply validated antibody pairs, recombinant proteins, and ancillary reagents to streamline your assay development from concept to clinic.