Anti-DDAH2 Rabbit Monoclonal Antibody for WB, ELISA - O95865

Core Product Specifications and Parameters

Biological Background: DDAH2 Function and Localization

• DDAH2 (Protein G6a, DDAHII) is an inactive member of the dimethylarginine dimethylaminohydrolase family that does not metabolize N(G),N(G)-dimethyl-L-arginine (ADMA) (PubMed:21493890, 37296100). Related references: PMID:21493890 PMID:37296100
• In endothelial cells, it induces vascular endothelial growth factor (VEGF) expression through PKA-dependent phosphorylation of transcription factor SP1, independent of nitric oxide (NO) and NO synthase (By similarity).
• It enhances pancreatic insulin secretion by upregulating secretagogin (SCGN), an insulin vesicle docking protein, via SP1-mediated transcriptional activation (By similarity).
• Upon viral infection, DDAH2 translocates to mitochondria and triggers mitochondrial fission through activation of DNM1L, thereby inhibiting MAVS-dependent innate immune responses (PubMed:33850055). Related references: PMID:33850055
• The protein localizes to both the cytoplasm and mitochondria.
• DDAH2 is widely expressed, with detection in heart, placenta, lung, liver, skeletal muscle, kidney, and pancreas, and at very low levels in brain.

Experimental Guidance and Technical Tips

• The immunogen comprises amino acids 1–100 at the N‑terminus of human DDAH2; verify that this region is present in the target isoform of interest.
• For Western blot, the predicted molecular weight is approximately 30 kDa; select an appropriate gel percentage and transfer protocol.
• The antibody cross-reacts with mouse and rat DDAH2, offering potential utility in comparative studies—validate in the relevant sample system.
• For ELISA, determine optimal coating concentration and assay parameters through standard optimization.
• Subcellular localization to cytoplasm and mitochondria may require fractionation or specific lysis buffers for detection of mitochondrial pools.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For targets such as DDAH2, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare-target sourcing to streamline your development workflow. Whether you are working in vascular biology, immunology, or metabolic research, CamelBio bridges your raw-material needs with supply-chain efficiency.