Anti-DDAH1 Rabbit Polyclonal Antibody for WB, IF/ICC and ELISA - O94760

Core Product Specifications and Parameters

Biological Background: DDAH1 Function and Localization

• DDAH1 (Dimethylarginine dimethylaminohydrolase 1) is a hydrolase enzyme that specifically hydrolyzes N(G),N(G)-dimethyl-L-arginine (ADMA) and N(G)-monomethyl-L-arginine (MMA), endogenous inhibitors of nitric oxide synthase (NOS). This activity plays a critical role in regulating nitric oxide (NO) generation.
• The DDAH1 gene undergoes alternative splicing, generating multiple isoforms that may exhibit tissue-specific expression or distinct regulatory properties.
• DDAH1 is a metal-binding protein that coordinates zinc ions, which are essential for its catalytic activity and structural integrity.
• Post-translational modifications of DDAH1 include acetylation and S-nitrosylation, which may modulate its enzymatic function and subcellular localization in response to cellular signals.
• DDAH1 is expressed at detectable levels in brain, liver, kidney, and pancreas, with lower expression in skeletal muscle, suggesting tissue-specific roles in NO-mediated processes.
• Keywords from UniProt: 3D-structure, Acetylation, Alternative splicing, Direct protein sequencing, Hydrolase, Metal-binding, Proteomics identification, Reference proteome, S-nitrosylation, Zinc.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein encompassing amino acids 1-285 of human DDAH1, which corresponds to a large N-terminal region. This design typically yields antibodies with good specificity for the target protein.
• For Western blotting, the predicted molecular weight is 31 kDa. Verify band size in positive control lysates (e.g., from human brain, liver, or kidney) and consider using appropriate loading controls.
• For immunofluorescence/immunocytochemistry, optimize fixation and permeabilization protocols to achieve specific staining, and validate with negative controls.
• Cross-reactivity with mouse and rat DDAH1 has been observed; however, due to sequence differences, it is recommended to confirm performance using lysates or tissue sections from the relevant species.
• ELISA applications may require optimization of coating buffers and detection systems; check the linear range using recombinant DDAH1 protein as a standard.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your comprehensive one-stop platform for IVD raw materials, offering reliable access to validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. For DDAH1-related research into nitric oxide regulation and cardiovascular or metabolic diseases, CamelBio can supply high-quality reagents tailored to your assay development needs. Our team supports diagnostic manufacturers, labs, and research institutes from concept to clinic, helping streamline your IVD product development.