Anti-DDAH1 Rabbit Monoclonal Antibody for WB and ELISA - O94760

Core Product Specifications and Parameters

Biological Background: DDAH1 Function and Localization

• DDAH1 is an enzyme that hydrolyzes asymmetric dimethylarginine (ADMA) and monomethylarginine (MMA), endogenous inhibitors of nitric oxide synthase (NOS), thereby regulating nitric oxide production.
• It belongs to the dimethylarginine dimethylaminohydrolase family and is also known as DDAHI or dimethylargininase-1.
• The DDAH1 gene undergoes alternative splicing, and the protein is post-translationally modified by acetylation and S-nitrosylation, which can affect its activity and stability.
• As a zinc-dependent hydrolase, DDAH1 requires metal binding for its catalytic function.
• DDAH1 is predominantly expressed in the brain, liver, kidney, and pancreas, with lower levels in skeletal muscle, consistent with its role in cardiovascular and renal physiology.

Experimental Guidance and Technical Tips

• For Western blotting, the antibody targets a sequence within amino acids 186-285 of human DDAH1; verify epitope conservation if using non-human, non-rat species.
• Use positive controls such as human liver or kidney lysates, and consider knockout validation to confirm specificity.
• When performing ELISA, optimize antibody concentration and include a peptide competition assay to confirm binding specificity.
• Due to low expression in certain tissues like skeletal muscle, sample enrichment or higher protein loading may improve detection in Western blot.
• The predicted molecular weight is ~31 kDa; use a 12% SDS-PAGE gel and appropriate transfer conditions for optimal resolution.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a one-stop sourcing partner for IVD raw materials, supporting diagnostic manufacturers and researchers from concept to clinic. For DDAH1 research in nitric oxide regulation and related disease areas, CamelBio provides validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents to accelerate your assay development. Contact us to explore customized raw-material solutions for your diagnostic needs.