Anti-DCTN5 Rabbit Polyclonal Antibody for WB, ELISA - Q9BTE1

Core Product Specifications and Parameters

Biological Background: DCTN5 Function and Localization

• DCTN5 encodes dynactin subunit 5 (also known as p25), a key component of the dynactin complex.
• As part of dynactin, DCTN5 activates cytoplasmic dynein for ultra-processive movement along microtubules, facilitating long-distance intracellular transport.
• DCTN5 is predominantly cytoplasmic and associates with the cytoskeleton. Additionally, it localizes to centromeres and kinetochores on chromosomes.
• The protein undergoes post-translational acetylation, revealed by mass spectrometry-based proteomics.
• Alternative splicing of the DCTN5 gene generates multiple protein isoforms.
• With a molecular weight of ~20 kDa (predicted 20.1 kDa), DCTN5 is a relatively small dynactin subunit essential for dynein motor function.

Experimental Guidance and Technical Tips

• For Western blot, a recommended starting dilution range is 1:500–1:2000, but optimization is advised based on your sample and detection system.
• Include appropriate positive controls (e.g., HeLa, HEK293, or NIH/3T3 lysates) and a negative control (knockdown or knockout cells) to confirm specificity.
• The polyclonal nature of this antibody may yield robust signal; however, lot-to-lot consistency should be verified by the end user.
• For ELISA, consider coating with recombinant DCTN5 or cell lysates and titrate both coating antigen and primary antibody for best signal-to-noise.
• The immunogen (N-terminal peptide) suggests the antibody recognizes the N-terminus of DCTN5; thus, epitope accessibility may be influenced by protein folding or complex formation. Test under native and denaturing conditions as needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated partner for IVD raw material sourcing, offering validated antibodies, recombinant proteins, and ancillary reagents for the dynactin and dynein motor research community. From polyclonal antibodies like this anti-DCTN5 to optimized monoclonal pairs and bulk reagents, we streamline procurement to accelerate your diagnostic and research projects. Contact our team for custom antibody production or rare target sourcing.