Anti-DCTN5 Monoclonal Antibody for WB, ELISA - Q9BTE1

Core Product Specifications and Parameters

Biological Background: DCTN5 Function and Localization

• DCTN5 encodes dynactin subunit 5, also known as p25, a component of the dynactin complex.
• The dynactin complex is essential for activating the molecular motor dynein, enabling ultra-processive transport of cargo along microtubules.
• Alternative splicing produces multiple isoforms, contributing to functional diversity (Keywords: Alternative splicing).
• Subcellularly, DCTN5 localizes to the cytoplasm and cytoskeleton, and is also found at centromeres and kinetochores during mitosis.
• Post-translational modifications include acetylation, which may regulate protein stability or interactions (Keywords: Acetylation).
• DCTN5 is part of the reference proteome and has been identified through proteomics analyses (Keywords: Proteomics identification, Reference proteome).

Experimental Guidance and Technical Tips

• For Western blot, the antibody detects a band near 20 kDa corresponding to endogenous DCTN5 in human, mouse, and rat samples.
• In ELISA, validate optimal coating concentrations and detection conditions for your specific assay format.
• The immunogen spans the full-length human DCTN5 protein (aa 1-182), suggesting it may recognize the native conformation; consider its use in immunoprecipitation after empirical validation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive range of IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and support for rare target sourcing. For researchers investigating intracellular transport or chromosome dynamics, CamelBio can facilitate procurement of DCTN5 antibodies and related dynactin complex reagents to accelerate your assay development from concept to clinic.