Anti-DCTN2 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q13561

Core Product Specifications and Parameters

Biological Background: DCTN2 Function and Localization

• Dynactin subunit 2 (DCTN2), also known as p50 dynamitin, RBP50, DCTN50, or DYNAMITIN, is a key component of the dynactin complex, which activates the molecular motor dynein for ultra-processive transport along microtubules.
• Within the dynactin shoulder domain, DCTN2 binds the ACTR1A filament and functions as a molecular ruler, determining the precise length of the complex (by similarity).
• DCTN2 modulates cytoplasmic dynein binding to organelles and plays a critical role in prometaphase chromosome alignment and spindle organization during mitosis.
• The protein is involved in anchoring microtubules to centrosomes, contributing to the structural integrity and function of the microtubule-organizing center.
• DCTN2 may also participate in synapse formation during brain development (by similarity).
• It localizes to the cytoplasm, cytoskeleton, centrosome, and membrane, reflecting its dynamic roles in intracellular transport and cell division.
• Post-translational modifications include acetylation and phosphorylation, and the protein features coiled-coil domains essential for its structural and functional interactions.
• Alternative splicing generates multiple isoforms, and the canonical protein has a molecular weight of approximately 44 kDa.

Experimental Guidance and Technical Tips

• The immunogen used corresponds to amino acids 50–320 of human DCTN2, targeting a region conserved across species and core functional domains; this design may confer broad reactivity.
• The antibody is indicated for WB, IF/ICC, and ELISA, covering common detection needs; consider validating in your specific sample system to confirm performance.
• Reported cross-reactivity with human and rat suggests utility in rodent models; for other species, assess sequence homology before use.
• Endogenous DCTN2 is detected near 44 kDa on Western blot; confirm band size using appropriate molecular weight markers and positive controls.
• For immunofluorescence, consider co-staining with centrosomal or microtubule markers to verify localization patterns.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio equips diagnostic manufacturers, laboratories, and research institutes with reliable one-stop access to IVD raw materials—from concept to clinic. This anti-DCTN2 antibody supports investigations into dynein-mediated transport and mitotic regulation, and we supply validated antibody pairs, optimized monoclonals/polyclonals, bulk ancillary reagents, and rare target raw materials to streamline your procurement. Let CamelBio be your sourcing partner for building robust assays with confidence.