Anti-DCTN2 Polyclonal Antibody for WB, ELISA - Q13561

Core Product Specifications and Parameters

Biological Background: DCTN2 Function and Localization

• DCTN2 (also known as dynamitin, p50, RBP50, DCTN50) is a subunit of the dynactin complex, a key activator of the molecular motor dynein.
• It functions in ultra-processive transport along microtubules, binding to the ACTR1A filament within the dynactin shoulder domain and acting as a molecular ruler to determine dynactin length.
• DCTN2 modulates cytoplasmic dynein binding to organelles and plays a critical role in prometaphase chromosome alignment and spindle organization during mitosis.
• It is involved in anchoring microtubules to centrosomes, contributing to cytoskeletal organization and cell division.
• The protein is localized to the cytoplasm, cytoskeleton, microtubule organizing center (centrosome), and membrane.
• DCTN2 is subject to post-translational modifications including acetylation and phosphorylation, and the gene undergoes alternative splicing.
• May also play a role in synapse formation during brain development.

Experimental Guidance and Technical Tips

• The immunogen used to generate this antibody spans a region within the coiled-coil domain of DCTN2, which may provide specificity for the native protein.
• For western blot (WB), validate performance in the appropriate cell or tissue lysate; a band near 44 kDa is expected.
• For ELISA, consider using recombinant DCTN2 as a positive control to confirm reactivity.
• This antibody cross-reacts with human, mouse, and rat DCTN2; however, optimal dilution and conditions should be determined in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a streamlined procurement pathway for IVD raw materials, bridging global suppliers and diagnostic developers. We supply validated antibody pairs, optimized polyclonal antibodies like this anti-DCTN2 reagent, and bulk ancillary reagents to accelerate your research in dynein transport and mitosis. Leverage our expertise to source rare targets and scale your assay development with confidence.