Anti-DCTD Polyclonal Antibody for WB, IHC-P, ELISA - P32321

Core Product Specifications and Parameters

Biological Background: DCTD Function and Localization

• DCTD encodes deoxycytidylate deaminase (dCMP deaminase), a homotetrameric zinc-dependent hydrolase that belongs to the cytidine and deoxycytidylate deaminase family.
• It catalyzes the deamination of dCMP to dUMP, providing the essential substrate for thymidylate synthase (TYMS) in de novo dTMP synthesis. Related references: PMID:7685356
• DCTD also mediates a nucleotide salvage pathway that eliminates epigenetically modified 5-hydroxymethyl-dCMP (hmdCMP) through deamination to cytotoxic hmdUMP. Related references: PMID:33833118
• The enzyme is allosterically regulated and undergoes phosphorylation, as evidenced by proteomics data.
• Alternative splicing generates multiple transcript variants, contributing to functional diversity.
• Key structural and functional keywords include 3D-structure, metal-binding (zinc), nucleotide biosynthesis, and direct protein sequencing.

Experimental Guidance and Technical Tips

• The immunogen spans the N-terminal 178 amino acids of human DCTD, ensuring robust detection across canonical isoforms.
• For western blotting, a band near 20 kDa is expected; validate the signal in your sample system and consider loading controls.
• In IHC-P, antigen retrieval (e.g., citrate buffer pH 6.0) may enhance staining; titrate the antibody for optimal signal-to-background ratio.
• For ELISA, this polyclonal antibody can serve as capture or detection reagent; consider pairing with a monoclonal antibody for sandwich formats.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio equips diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials, from validated antibody pairs and optimized polyclonal/monoclonal antibodies to bulk ancillary reagents. This anti-DCTD antibody supports studies in pyrimidine metabolism and epigenetic regulation, and our team can assist with custom sourcing for rare or specialized targets. Partner with CamelBio to streamline your development from concept to clinic.