Anti-DCT Polyclonal Antibody for WB, ELISA - P40126

Core Product Specifications and Parameters

Biological Background: DCT Function and Localization

• DCT (TRP-2/TYRP2) is a melanocyte-specific enzyme belonging to the tyrosinase-related protein family.
• Catalyzes the isomerization of L-dopachrome to 5,6-dihydroxyindole-2-carboxylic acid (DHICA) in the melanin biosynthesis pathway. Related references: PMID:33100333
• Contains a signal peptide and a single transmembrane domain, localizing primarily to the melanosome membrane.
• Post-translational modifications include glycosylation, and the enzyme binds zinc as a cofactor.
• Alternative splicing generates multiple transcript variants.
• Mutations in DCT are associated with oculocutaneous albinism type 8 (OCA8).

Experimental Guidance and Technical Tips

• For Western blot analysis, consider that the expected band size is approximately 59 kDa. Validate detection in the relevant sample system under reducing conditions.
• This polyclonal antibody is suitable for ELISA applications; appropriate controls and optimization of coating and detection conditions should be performed in the target matrix.
• The immunogen corresponds to amino acids 50–350 of human DCT; cross-reactivity with mouse DCT is specified, but cross-reactivity with other species or isoforms should be confirmed experimentally.

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At CamelBio, we streamline your IVD raw-material sourcing for melanogenesis and pigmentation research. Beyond anti-DCT polyclonal antibodies, we offer optimized monoclonal antibodies, validated antibody pairs, and bulk ancillary reagents to support assay development. Let our sourcing expertise bridge your concept to clinic.