Anti-DcR1/CD263 Rabbit Monoclonal Antibody for WB, IHC-P, FC, ELISA - O14798

Core Product Specifications and Parameters

Biological Background: DcR1/CD263 Function and Localization

• DcR1/CD263, encoded by the TNFRSF10C gene, is a tumor necrosis factor receptor superfamily member also known as TRAIL-R3, LIT, and TRID. It functions as an antagonist decoy receptor for the cytotoxic ligand TRAIL (Apo-2L).
• Unlike the functional TRAIL receptors TNFRSF10A and TNFRSF10B, DcR1 lacks an intracellular death domain. By competing for TRAIL binding, it inhibits apoptosis and protects cells from TRAIL-induced cell death.
• The protein is anchored to the cell membrane via a glycophosphatidylinositol (GPI) linkage and contains a signal peptide, multiple cysteine-rich repeat domains, and one or more disulfide bonds.
• DcR1 is expressed at higher levels in normal tissues compared to tumor cell lines, with prominent expression in peripheral blood lymphocytes, spleen, skeletal muscle, placenta, lung, and heart.
• Keywords from UniProt include apoptosis, cell membrane, disulfide bond, glycoprotein, GPI-anchor, lipoprotein, receptor, and signal, reflecting its structural and functional features.
• Post-translational modifications include N-linked glycosylation and the GPI-anchor attachment, which are critical for membrane localization and stability.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (amino acids 26-235) of human DcR1/CD263, suggesting the antibody recognizes the ligand-binding region of both membrane-bound and soluble forms.
• For Western blotting, a band near 27 kDa is expected under reducing conditions. Due to potential glycosylation, consider testing samples in both reduced and non-reduced states to optimize detection.
• For flow cytometry, use live, unfixed cells to preserve surface epitope accessibility. Titrate the antibody to determine the optimal staining concentration.
• In immunohistochemistry (paraffin-embedded), antigen retrieval may be required. Validate staining specificity by comparing with known positive (e.g., normal spleen) and negative controls.
• For ELISA, this antibody can be used as a capture or detection reagent. Pair it with a non-competing clone for sandwich ELISA development.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For apoptosis research involving TRAIL decoy receptor DcR1/CD263, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. Our supply capabilities extend to rare-target raw-material sourcing to support your assay development needs.