Anti-DcR1/CD263 Rabbit Monoclonal Antibody for WB, ELISA - O14798

Core Product Specifications and Parameters

Biological Background: DcR1/CD263 Function and Localization

• DcR1/CD263 (TNFRSF10C) is a tumor necrosis factor receptor superfamily member that acts as a decoy receptor for the cytotoxic ligand TRAIL. It lacks the intracellular death domain required for apoptosis induction.
• By competing with functional death receptors TRAIL-R1 (TNFRSF10A) and TRAIL-R2 (TNFRSF10B) for ligand binding, it protects cells from TRAIL-induced apoptosis.
• Alternative names include LIT, DCR1, TRID, TRAILR3, and CD263, reflecting its role as a lymphocyte inhibitor of TRAIL and a TRAIL receptor without an intracellular domain.
• The protein localizes to the cell membrane and is attached via a GPI anchor.
• It undergoes post-translational modifications including disulfide bond formation and glycosylation.
• Predicted molecular weight is approximately 27.4 kDa (UniProt), consistent with the observed migration at 27 kDa.
• Tissue expression is higher in normal tissues (peripheral blood lymphocytes, spleen, skeletal muscle, placenta, lung, heart) compared to tumor cell lines, suggesting a role in protecting normal cells from TRAIL-induced apoptosis.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 150-230 within the extracellular domain of human DcR1. This region is likely accessible on the cell surface, supporting detection of the membrane-bound receptor.
• For Western blot, consider testing both reducing and non-reducing conditions to account for disulfide bonds. The expected band is around 27 kDa.
• When performing ELISA, validate the antibody with recombinant TRAIL ligand or receptor-specific standards to confirm binding specificity.
• Given that DcR1 is a GPI-anchored glycoprotein, solubilization and sample preparation protocols may influence detection; optimization with appropriate detergents is advised.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research laboratories a streamlined platform for IVD raw material procurement, from concept to clinic. This anti-DcR1/CD263 monoclonal antibody supports apoptosis research and biomarker development, exemplifying our commitment to supplying high-quality reagents. Complement your workflow with our validated antibody pairs, bulk ancillary reagents, and rare target sourcing capabilities to accelerate your diagnostic assay development.