Anti-DCP2 Rabbit Polyclonal Antibody for WB, ELISA - Q8IU60

Core Product Specifications and Parameters

Biological Background: DCP2 Function and Localization

• DCP2 (NUDT20) is an m7GpppN-mRNA hydrolase, also known as nucleoside diphosphate-linked moiety X motif 20, that functions as a decapping metalloenzyme and is also referred to as hDpc or Nudix motif 20.
• DCP2 catalyzes the cleavage of the 7-methyl guanine cap structure on mRNA, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP (PubMed:12486012, PubMed:12923261, PubMed:21070968). It has higher activity towards non-polyadenylated mRNAs and requires an RNA moiety for cap recognition (PubMed:21070968).
  Related references: PMID:12486012, PMID:12923261, PMID:21070968
• DCP2 is necessary for both normal mRNA turnover and nonsense-mediated mRNA decay (PubMed:14527413).
  Related references: PMID:14527413
• It plays a role in replication-dependent histone mRNA degradation (PubMed:18172165).
  Related references: PMID:18172165
• The presence of a N6-methyladenosine (m6A(m)) cap at the second transcribed position provides resistance to DCP2-mediated decapping (PubMed:28002401).
  Related references: PMID:28002401
• DCP2 blocks autophagy under nutrient-rich conditions by repressing ATG-related gene expression through transcript degradation (PubMed:26098573).
  Related references: PMID:26098573
• Subcellularly, DCP2 is found in the cytoplasm, P-bodies, and nucleus. It is expressed in brain and testis but not detected in heart at the protein level.
• Keywords associated with DCP2 include alternative splicing, metal-binding, phosphoprotein, and RNA-binding, reflecting its regulation and interactions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-240) of human DCP2. This polyclonal antibody may recognize multiple isoforms due to alternative splicing; validation in the relevant sample system is recommended.
• For Western blot, a band around 48 kDa (predicted molecular weight) is expected. Given DCP2's expression in brain and testis, lysates from these tissues or cell lines derived from them are suitable positive controls.
• In ELISA, the antibody may be used for detecting recombinant or endogenous DCP2; consider optimizing coating and detection conditions.
• As a polyclonal antibody, cross-reactivity with other Nudix family members is possible; inclusion of appropriate controls is advised.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop sourcing partner for IVD raw materials, supporting diagnostic manufacturers and research institutions from concept to clinic. This anti-DCP2 polyclonal antibody is well-suited for studying mRNA decapping and post-transcriptional regulation. Through CamelBio's extensive network, customers can access validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and even rare-target raw-material sourcing to streamline assay development and production.