Anti-DCP1B Polyclonal Antibody for WB, ELISA - Q8IZD4

Core Product Specifications and Parameters

Biological Background: DCP1B Function and Localization

• DCP1B (mRNA-decapping enzyme 1B) is a hydrolase implicated in mRNA degradation pathways, including both general mRNA turnover and nonsense-mediated decay (NMD).
• The enzyme is thought to catalyze the removal of the 5' 7-methylguanosine cap from mRNA, generating 5'-phosphorylated mRNA and 7m-GDP, a critical step in mRNA decay.
• DCP1B is localized to both the cytoplasm and the nucleus, suggesting roles in multiple subcellular compartments.
• Post-translational modifications such as acetylation and phosphorylation have been identified, indicating potential regulatory mechanisms controlling its activity or localization.
• Alternative splicing of the DCP1B gene produces multiple transcript variants, though their functional significance remains to be fully characterized.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 386–617 of human DCP1B; therefore, the antibody recognizes a region towards the C-terminus. Consider using the recombinant immunogen as a positive control in ELISA.
• For Western blot, the predicted molecular weight is approximately 68 kDa; validate band specificity by comparing with known controls or knockdown samples.
• Cross-reactivity with rat is indicated, likely due to sequence conservation in the immunogen region. Verify performance in rat samples using appropriate tissue or cell lysates.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive B2B partner for IVD raw materials, supporting diagnostic manufacturers and researchers from concept to clinic. This DCP1B antibody is part of our portfolio of mRNA metabolism research tools, and we can also supply validated antibody pairs, bulk ancillary reagents, and sourcing services for rare targets. Let CamelBio streamline your procurement of optimized polyclonal and monoclonal antibodies for your next diagnostic development project.